Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (2): 257-260.doi: 10.3969/j.issn.1673-8225.2012.02.015

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Establishment and electrophysiology evaluation of an animal model of 3/4 crosscut spinal cordinjury

Li Jing-de1, Lu Pei-gang2   

  1. 1General Hospital ofJinan Military AreaCommand of ChinesePLA (PostgraduateBasis of LiaoningMedical University),Jinan 250031,Shandong Province,China;
    2Departmentof Neurosurgery,General Hospital ofJinan Military AreaCommand of ChinesePLA, Jinan 250031,Shandong Province,China
  • Received:2011-08-25 Revised:2011-10-26 Online:2012-01-08 Published:2012-01-08
  • Contact: Lu Pei-gang, Doctor,Associate professor,Associate chiefphysician, Master’ssupervisor,Department ofNeurosurgery,General Hospital ofJinan Military AreaCommand of ChinesePLA, Jinan 250031,Shandong Province,China pglu912@126.com
  • About author:Li Jing-de★,Studying for master’sdegree, Physician,General Hospital ofJinan Military AreaCommand of ChinesePLA (PostgraduateBasis of LiaoningMedical University),Jinan 250031,Shandong Province,China lijingde1981@163.com

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Abstract:

BACKGROUND: Establishing a stable and standard animal model of spinal cord injury is a precondition to investigate the repairof spinal cord injury.
OBJECTIVE: To establish a practical, standard and dependable model of acute spinal cord injury in rats.
METHODS: A total of 60 female Wistar rats were randomly assigned into two groups, spinal cord injury group and control group.The spinal cord corresponding to T8 thoracic vertebrae of spinal cord injury rats were open. The dura mater was sheared opening.The posterior 3/4 of the spinal cord was cut across the spinal dorsal midline by a specially designed Venus eye scissors, 1.5 mmin depth. The sensory evoked potentials and motor evoked potentials were examined in an hour. As for the control rats, thevertebral lamina and dura mater were opened. The spinal cord was exposed for sham operation.
RESULTS AND CONCLUSION: The success rate of model construction was 100%. The function recovery of control group wasclose to normal in the 1st week after model establishment. The function recovery of spinal cord injury group started in the 2nd weekafter model establishment and virtually stopped in the 4th week. The final motor-function grades were no more than 10. There wassignificant difference between the two groups. The wave amplitude of sensory evoked potentials and motor evoked potentials inspinal cord injury group decreased sharply and significantly, the latency extended significantly (P < 0.01). These findings indicatethat the establishment of rat acute spinal cord injury model of 3/4 traumatic transection is easy and reproducible. It is an idealmodel for the investigation of spinal regenerative repair.

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