Abstract:

BACKGROUND: Vascular endothelial growth factor and angiopoietin-1 play an important role in the initiating and ongoingprocess of angiogenesis. Study on the effects of vascular endothelial growth factor and angiopoietin-1 on vascular endothelialcells is significant.
OBJECTIVE: To explore the effect of vascular endothelial growth factor and angiopoietin-1 on the transfer and proliferation abilityin rat vascular endothelial cells and to explore its mechanism on angiogenesis.
METHODS: Rat umbilical vein endothelial cells were treated with vascular endothelial growth factor and angiopoietin-1respectively or in combination. Scratch test and MTT assay were used to detect the cell transfer and proliferation ability. Themorphology, activity and transfer ability of endothelial cells were observed.
RESULTS AND CONCLUSION: According to scratch test, there was no significant difference in cell transfer ability between thecells treated with vascular endothelial growth factor alone and the control cells. The cell transfer ability after treated withangiopoietin-1 individually did not increase; on the contrary, it was weakened compared with that of the control group. Thetransfer ability in cells treated with vascular endothelial growth factor combined with angiopoietin-1 improved significantlycompared with that of the control group. According to MTT assay, neither vascular endothelial growth factor nor angiopoietin-1was found to improve the proliferation ability in rat umbilical vein endothelial cells. Combination of vascular endothelial growthfactor and angiopoietin-1 significantly improved the proliferation of rat umbilical vein endothelial cells. These findings indicate thatthe transfer ability, proliferation ability and angiogenesis in rat umbilical vein endothelial cells are only improved by thecombination of vascular endothelial growth factor and angiopoietin-1.