Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (2): 239-242.doi: 10.3969/j.issn.1673-8225.2012.02.011

Previous Articles     Next Articles

Xingnaojing inhibits the proliferation of human umbilical cord vein endothelial cells mediated byrecombinant human tumor necrosis factor

Ouyang Hai-chun1, Wu Wo-dong2, Zhong Dong-mei3, Wu Yan-xian1, Li Wen-jie2, Chen Xi-ming2   

  1. 1Department ofCardiology, the FirstPeople’s Hospital ofShunde District,Foshan 528300,Guangdong Province,China; 2Departmentof Cardiology, 3Department ofChinese TraditionalMedicine, the ThirdAffiliated Hospital ofGuangzhou MedicalUniversity,Guangzhou510150, GuangdongProvince, China
  • Received:2011-06-04 Revised:2011-08-29 Online:2012-01-08 Published:2012-01-08
  • Contact: Wu Wo-dong, Chiefphysician,Department ofCardiology, the ThirdAffiliated Hospital ofGuangzhou MedicalUniversity,Guangzhou510150, GuangdongProvince, China wuwodong@hotmail.com
  • About author:Ouyang Hai-chun★,Master, Physician,Department ofCardiology, the FirstPeople’s Hospital ofShunde District,Foshan 528300,Guangdong Province,China oyhc99004@hotmail.com
  • Supported by:
     Integrated Traditionaland WesternMedicine ResearchProjects ofGuangzhou MunicipalTraditional ChineseMedicine Bureau, No.2004A014*;Research Projects ofthe Construction forTraditional ChineseMedicine Province toGuangzhou MunicipalTraditional ChineseMedicine Bureau, No.2060063*

PDF

402

Abstract:

BACKGROUND: Xingnaojing (XNJ) water solubility intravenous injection is one of prescription similar with Angong Niuhuang pillwhich has been a most great reputation Chinese traditional medicine. Xingnaojing is used in clinical treatment for coronary heartdisease patients and has achieved good results. However, the mechanism has not been cleared.
OBJECTIVE: To investigate the inhibition effects of XNJ on the proliferation of human umbilical vein endothelial cells (HUVECs)mediated by recombinant human tumor necrosis factor α (rhTNF-α). To evaluate the value of XNJ on the proliferation of vascularendothelial cells impaired.
METHODS: The passage 3-5 vein endothelial cells were obtained, and were induced to proliferate by adding 10 μg/L rhTNF-α inthe culture medium. XNJ in different concentration were injected in the XNJ group, fluvastatin as positive control group, and blankcontrol group had been respectively incubated with HUVECs mediated by rhTNF-α.
RESULTS AND CONCLUSION: The endochylema was shrinked, adherent cells were decreased and necrosis cells wereincreased in HUVECs of XNJ group under inverted phase microscope. The growth rate of HUNVCs proliferation was significantlylower in XNJ group in each different concentration than that in control group (P < 0.05), and showed dose and time dependentmanner. XNJ can effectively inhibit the proliferation of HUVECs mediated by rhTNF-α.

CLC Number: