Abstract:

BACKGROUND: Recent study shows that the adenosine receptor agonists can promote the collagen synthesis, and theadenosine receptor antagonists can inhibit the collagen synthesis and reduce the proliferation of skin collagen fiber. Theexpression of transforming growth factor β (TGF-β) in hypertrophic scar of adenosine A2A knockout mice models is decreased.
OBJECTIVE: To observe the changes of collagens in hypertrophic scars of adenosine receptor A2A knockout mice and itsmechanism by picric acid-sirius red polarization method.
METHODS: The models of hypertrophic scars were made by adenosine A2A knockout mice and wild-type mice. The characterand the distribution of the collagen in the hypertrophic scars were observed by picric acid-sirius red polarization method, and thetype of the collagen, distribution, arrangement and content was confirmed.
RESULTS AND CONCLUSION: A large amount of eosinophilic collagen protein fibers were observed under polarizingmicroscope in the hypertrophic scars of wild-type control group. Type Ⅰ collagen fibers were in red and compact bunchiness andexhibited strong double refraction, the hypertrophic scars of adenosine A2A knockout mice were lack of thick collagen bundlesand was in sparse bunchiness, and the collage bundles were well-arranged and well-distributed. Compared with the wild-typecontrol group, adenosine A2A knockout mice showed significantly lower typeⅠcollagen fibers level (P < 0.01), as well as thehypertrophic scars. It indicated that adenosine A2A receptors played an active role in the proliferation of scars and could preventthe proliferation of scars.