Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (2): 227-230.doi: 10.3969/j.issn.1673-8225.2012.02.008

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Inhibitory effects of Bawei Rougan powder on chondrocytes apoptosis in rabbits withosteoarthritis

Liu Miao, Chen Shi-rong, Liu Wei, Xu Xi-dong, Si Hai-bo   

  1. Second AffiliatedHospital, ChongqingMedical UniversityChongqing 400010,China
  • Received:2011-10-19 Revised:2011-12-07 Online:2012-01-08 Published:2012-01-08
  • Contact: Chen Shi-rong,Associate professor,Second AffiliatedHospital, ChongqingMedical UniversityChongqing 400010,China chensr128@126.com
  • About author:Liu Miao★, Studyingfor master’s degree,Second AffiliatedHospital, ChongqingMedical UniversityChongqing 400010,China blue_ivyer@sina.com
  • Supported by:
    Traditional ChineseMedicine ResearchProgram of SecondAffiliated HospitalChongqing MunicipalHealth Bureau in2010, No. 2010-2-37*

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Abstract:

BACKGROUND: Traditional Chinese Medicine (TCM) has analgesic effects on osteoarthritis, but whether TCM can protectarticular cartilage and through which mechanism are rarely reported.
OBJECTIVE: To observe the effects of Bawei Rougan powder on chondrocytes apoptosis in rabbits with osteoarthritis.
METHODS: New Zealand adult white rabbits were randomly divided into three groups: normal, model, and treatment groups.Osteoarthritis models were established in model and treatment groups according to Hurth technique. At 1 week after modeling,rabbits in the normal and model groups were treated with saline solution, and the treatment group was treated with the powderextract. At 8 weeks after modeling, characteristics of cartilage histological were observed by Mankin method. Activity of nitricoxide (NO) and inducible nitric oxide synthase (iNOS) in synovial fluids were measured by nitrate reductase assay, and apoptosisindex of chondrocytes in all groups were measured by TUNEL ( TdT-mediated biotinylated-dUTP nick end labling method).
RESULTS AND CONCLUSION: Compared with the model group, Mankin's score, activity of NO and iNOS and apoptosis indexof chondrocytes were decreased in the treatment group (P < 0.05). It is confirmed that Bawei Rougan powder can suppress theapoptosis of chondrocytes in rabbits with osteoarthritis．

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