Abstract:

BACKGROUND: Previous studies showed that insulin-like growth factor-1 (IGF-1) and platelet-derived growth factor (PDGF) cansuppress apoptosis of human intervertebral cells.
OBJECTIVE: To observe the effects of IGF-1 and PDGF on the biological potential of the human degenerative NP cells in vitro.
METHODS: Human degenerative nucleus pulposus cells were cultured in monolayer in vitro and identified byimmunohistochemical staining. The cells of passage 3 were treated separately with different growth factors and then randomlydivided into 4 groups: IGF-1 group, PDGF group, IGF-1+PDGF group and control group.
RESULTS AND CONCLUSION: IGF-1 and PDGF could stimulate cell proliferation and synthesis of collagen type Ⅱ andaggrecan, and IGF-1 raised more content of collagen typeⅡ than the PDGF (P < 0.05), but the PDGF raised more content ofaggrecan than the IGF-1 (P < 0.05). IGF-1 stimulated the synthesis of collagen typeⅠ, then PDGF inhibited the synthesis. Boththe IGF-1 and PDGF can stimulate cell proliferation, the synthesis of collagen type Ⅱ and aggrecan, thereby enhance thebiological potential of the human degenerative nucleus pulposus cells.