Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (2): 206-210.doi: 10.3969/j.issn.1673-8225.2012.02.003

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Ultrastructure changes of dog dental pulp cells transfected with bone morphogenetic proteins 2 in vitro

Feng Yan-hong1, Diao Zhi-hong2, Gao Yi1, Li Wei1   

  1. 1Department ofStomatology, HebeiProvincial Hospital ofTraditional ChineseMedicine,Shijiazhuang050011, HebeiProvince, China;
    2First Hospital ofShijiazhuang,Shijiazhuang050011, HebeiProvince, China
  • Received:2011-11-02 Revised:2011-11-10 Online:2012-01-08 Published:2012-01-08
  • Contact: Gao Yi, Master, Chiefphysician, Professor,Department ofStomatology, HebeiProvincial Hospital ofTraditional ChineseMedicine,Shijiazhuang050011, HebeiProvince, China dzh666888@yahoo.cn
  • About author:Feng Yan-hong,Attending physician,Department ofStomatology, HebeiProvincial Hospital ofTraditional ChineseMedicine,Shijiazhuang050011, HebeiProvince, China hbedzz@sohu.com Diao Zhi-hong,Associate chiefphysician, FirstHospital ofShijiazhuang,Shijiazhuang050011, HebeiProvince, China. dzh66888@yahoo.cn Feng Yan-hong andDiao Zhi-hong wereconsidered as co-firstauthors.
  • Supported by:

    Support Program ofScience andTechnologyDepartment of HebeiProvince, No.JB00-09276102D-15*

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Abstract:

BACKGROUND: The research has shown that bone morphogenetic protein 2 (BMP-2) gene can induce mesenchymal stem cellsdifferentiate into osteoblast phenotype and has the potential to the formation of new bone and reparative dentin.
OBJECTIVE: To investigate the progress of dog dental pulp cells (DDPCs) transfected with BMP-2 differentiate into odontoblastthrough the analysis of ultrastructure.
METHODS: The forth generation of DDPCs with stable traits was divided into three groups: Gene transfection group wastransfected with pEGFP-N1-BMP-2; Empty vector transfection group was transfected with EGFP-N1 empty fluorescence vector;Control group was without transfection.
RESULTS AND CONCLUSION: pEGFP-N1-BMP-2 eukaryotic expression plasmid was constructed successfully and transfectedwith DDPCs. pEGFP-N1-BMP-2 plasmid transfection could promote the secretion of BMP-2 in BMP2-DDPCs and the activity ofalkaline phosphatase of DDPCs. The transmission electron microscope observation showed that the transfected DDPCs had thephenotype characteristics of odontoblast. It indicates that the DDPCs transfected with pEGFP-N1-BMP-2 eukaryotic expressionplasmid has the characteristics of odontoblast.

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