Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (2): 201-205.doi: 10.3969/j.issn.1673-8225.2012.02.002

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Effect of bionic tiger-bone powder on rat osteoblasts proliferation and typeⅠcollagenexpression

Han Na1, Wang Tian-bing2, Kou Yu-hui2, Zhang Dian-ying2, Zhang Pei-xun2, Yin Xiao-feng2, Jiang Bao-guo2   

  1. 1Institute of Clinical Molecular Biology;   2Department of Orthopaedics and Traumatology, People's Hospital of Peking University, Beijing  100044, China；
  • Received:2011-09-21 Revised:2011-11-04 Online:2012-01-08 Published:2012-01-08
  • Contact: Jiang Bao-guo, Doctor, Chief physician, Doctoral supervisor, Department of Orthopaedics and Traumatology, People's Hospital of Peking University, Beijing 100044, China jiangbaoguo@vip.sina.com
  • About author:Han Na☆, Doctor, Assistant researcher, Institute of Clinical Molecular Biology, People's Hospital of Peking University, Beijing 100044, China hanna@bjmu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30973066*, 31040043*; Fund of Capital Medical Development and Research, No. 2007-2011*; Beijing Natural Science Foundation, No. 7113170*; Doctoral Fund of Ministry of Education of China, No. 20090001120096*; Peking University People’s Hospital Research and Development Funds, No. RDB2010-30*

     

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Abstract:

BACKGROUND: Bionic tiger-bone powder is mainly used for the treatment of osteoporosis, fracture, rheumatoid arthritis and other bone diseases, and has achieved better clinical efficacy. However, the mechanisms of osteogenesis has not clear yet.
OBJECTIVE: To observe the effects of bionic tiger-bone powder on the proliferation of rat osteoblasts and the expression of typeⅠcollagen.
METHODS: We cultivate primary osteoblasts with secondary enzyme digestion of the newborn rat skull bone tissue. The Concentrations of culture medium in bionic tiger-bone powder intervention group were 10-4, 10-5 and 10-6 g / L. The control group was injected with the serum-free DMEM culture medium without bionic tiger-bone powder.  
RESULTS AND CONCLUSION: MTT colorimetric assay results showed that bionic tiger-bone powder could improve osteoblasts proliferation vitality apparently (P < 0.05). Meanwhile the proportion of S phase and M/G2 phase cells was increased but that of G0/G1 phase cells was decreased through flow cytometry. The RT-PCR and ELISA test showed that bionic tiger-bone powder could increase the expression of osteoblasts typeⅠcollagen (P < 0.05) when handling with them in the concentration of 10-6, 10-5 and 10-4 mg/ml for 48 hours. Bionic tiger-bone powder could promote the proliferation of rat primary osteoblasts in vitro, and could promote the synthesis and secretion of typeⅠcollagen.