Chinese Journal of Tissue Engineering Research

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Human bone marrow mesenchymal stem cells transplantation induces neural regeneration and improves the recovery of nerve function in a rat stroke model

Liu Tai-yun1, Xiong Fu2, Lin Jun1, Wang Chuan-Sen1, Zhang Cheng3    

  1. 1Department of Neurology, Guizhou Provincial Hospital, Guiyang  550002, Guizhou Province, China; 2Insititute of Physiology, Southern Medical University, Guangzhou  510515, Guangdong Province, China; 3Department of Neurology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, China
  • Received:2011-11-04 Revised:2011-12-07 Online:2012-01-01 Published:2012-01-01
  • Contact: Liu Tai-yun, Department of Neurology, Guizhou Provincial Hospital, Guiyang 550002, Guizhou Province, Chinataiyunliu@163.com
  • About author:Liu Tai-yun☆, Doctor, Chief physician, Department of Neurology, Guizhou Provincial Hospital, Guiyang 550002, Guizhou Province, China taiyunliu@163.com
  • Supported by:

     Fund of Guizhou Health Department, No. [2006]130*, [2007]119-23*

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Abstract:

BACKGROUND: Studies have shown that allograft of bone marrow mesenchymal stem cells (BMSCs) or neural stem cells (NSCs) can improve the nerve function of rat stroke model.  
OBJECTIVE: To investigate survival and migration of BMSCs in part of the infarction region of transplanted rat and the neural regeneration. 
METHODS: Human BMSCs were transplanted into an immunosuppressed rat model of focal cerebral ischemia by vein. Behavioral recovery (neurological severity scores, NSS; rotarod test) and nerve function were also observed once a week. The rat were euthanized at 1-8 weeks after transplantation, and the expression of human nuclear antigen (HNA), marker of neural stem cells (Nestin) and nerve cell markers (Neuronal nuclei, cerebral tubulin, glial fibrillary acidic protein) were analyzed by immunohistochemistry staining, immunofluorescence staining and RT-PCR. 
RESULTS AND CONCLUSION: At 2 weeks after BMSCs transplantation, the NSS score of rat stroke model was decreased significantly (P < 0.05), and the residence time of the rotarod test was significantly prolonged. At 1-8 weeks after BMSCs transplantation, HNA+ cells were identified in subependymal region, hippocampus dentate gyrus and the site around the infarction. At 1 and 2 weeks after BMSCs transplantation, Nestin positive cells and mRNA expression could be seen in subependymal region, hippocampus dentate gyrus (CA1, CA3) and the site around the infarction, and lasted for 8 weeks. Two weeks later, HNA, cerebral tubulin positive cells and mRNA expression could be seen in subependymal region, hippocampus dentate gyrus and the site around the infarction. At 4-8 weeks after BMSCs transplantation, a small amount of glial fibrillary acidic protein positive cells and mRNA expression could be seen in subependymal region, hippocampus dentate gyrus and the site around the infarction. The data indicate that human BMSCs can survive and migrate to focal cerebral ischemia of a rat stroke model by intravenous delivery, then differentiate into nerve cells, promote the formation of nerve cells and improve the recovery of neurological function.

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