Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 65-69.doi: 10.3969/j.issn.1673-8225.2012.01.014

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Effect of nerve growth factor and epidermal growth factor on proliferation of endogenous neural stem cells after traumatic brain injury

Liu Yang1, Liu Wei-ping2, Wang Xiao-an2, Yang Yang2, Long Qian-fa2, Zheng Zhao-hui2, Zhang Yu-qi2, Zhong Jun2   

  1. 1Department of Neurosurgery, Third People’s Hospital of Mianyang City, Mianyang  621000, Sichuan Province, China; 2Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi’an  710032, Shaanxi Province, China
  • Received:2011-04-13 Revised:2011-06-21 Online:2012-01-01 Published:2012-01-01
  • Contact: Liu Wei-ping, Professor, Doctoral supervisor, Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, Chinaliuwpfmmu@163.com
  • About author:Liu Yang, Associate chief physician, Department of Neurosurgery, Third People’s Hospital of Mianyang City, Mianyang 621000, Sichuan Province, China liu10012002@sina.com
  • Supported by:

    the Fund from Sichuan Health Bureau, No. 2010-100301*
       

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Abstract:

BACKGROUND: Epidermal growth factor (EGF) and nerve growth factor (NGF) can promote the proliferation of neural stem cells, but their effects on endogenous stem cells after a traumatic brain injury in adult rats are studied little.
OBJECTIVE: To investigate the effect of NGF and EGF on proliferation of endogenous neural stem cells after traumatic brain injury in rats.
METHODS: Forty-eight adult SD rats were divided into three treatment groups and one control group at random, and the traumatic brain injury model was established with improved Feeney’s free-fall impacting method. Separate and combined injection of NGF and EGF were given to rats at 24 hours after traumatic brain injury. Normal saline was administrated into the rats in the control group.
RESULTS AND CONCLUSION: The scores of forelimb placing test and beam balance test examines in the NGF, EGF or the combination group were superior to those of the control group, and the scores in the combination group were better than those in the NGF or EGF group (P < 0.05). Compared with the control group, BrdU-labelde cells were detected in the bilateral subependymal zones, the dentate gyrus of the hippocampus and damage region after traumatic brain injury in NGF, EGF or the combination treatment group increased more significantly; the expression in the combination group was better than in the NGF or EGF group (P < 0.05). Proliferation of endogenous neural stem cells and restoration of limbs function after traumatic brain injury can be promoted by NGF or EGF. Morever, this effect can be improved more significantly by the combination of NGF and EGF.
 

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