Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3534-3537.doi: 10.3969/j.issn.1673-8225.2011.19.027

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Effect of olfactory ensheathing cells transplantation on the expression of transforming growth factor beta in the motor cortex of rats subjected to spinal cord transection

Li Yun1, Peng Chun2, Wang Ting-hua3   

  1. 1Department of Neurology, the Affiliated Hospital of Dali University, Dali  671000, Yunnan Province, China
    2Department of Technology, the Public Security Bureau of Dali Prefecture, Dali  671000, Yunnan Province, China
    3Institute of Neuroscience, Kunming Medical University, Kunming  650031, Yunnan Province, China
  • Received:2010-11-20 Revised:2011-01-19 Online:2011-05-07 Published:2011-05-07
  • Contact: Wang Ting-hua, Doctor, Professor, Doctoral supervisor, Institute of Neuroscience, Kunming Medical University, Kunming 650031, Yunnan Province, China tinghua_neuron@263.net
  • About author:Li Yun☆, Doctor, Attending physician, Department of Neurology, the Affiliated Hospital of Dali University, Dali 671000, Yunnan Province, China dlhliyun@163.com

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Abstract:

BACKGROUND: Many studies have showed that olfactory ensheathing cells (OECs) transplantation can result in significant functional improvements in rats subjected to spinal cord injury, but the underlying molecular mechanisms is not unknown.
OBJECTIVE: To observe the effect of OECs transplantation on the expression of transforming growth factor beta (TGF-β) mRNA in the motor cortex of the rats subjected to spinal cord transection.
METHODS: OECs isolated from transgenic mice expressing GFP and cultured by enzymatic digestion were made into cell suspension. Model of spinal cord transection (SCT) was established by cut transversely Sprague-Dawley (SD) rats T9 segment. After modeling, the rats were divided into sham-operation group, operation group and OECs transplantation group. RT-PCR was employed to detect gene expression of TGF-β. β-actin was used as control.
RESULTS AND CONCLUSION: RT-PCR results showed that compared with sham-operation group, the gene expression of TGF-β in the motor cortex increased significantly at 3 days postoperatively in the operation group (P < 0.05), and it returned to the level of sham-operation group at 7, 14, 21, and 28 days after modeling. Following OECs transplanatation, the expression of TGF-β in motor cortex at 21 days postoperatively showed a significant decrease compared with the operation group (P < 0.05). The findings indicate that spinal cord transection results in the up-regulation of the expression of TGF-β mRNA in the motor cortex in early period. OECs engrafted can decrease the expression of TGF-β mRNA in the later time so as to promote the recovery of spinal cord injury.

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