Abstract:

BACKGROUND: GATA-1, GATA-2, peroxisome proliferator-activated receptor gamma (PPAR-γ), and SCF gene play an important role in bone marrow hematopoietic and concomitant regulation mechanism.
OBJECTIVE: To observe the expressions of GATA-1, GATA-2, PPAR-γ, SCF gene in bone marrow mesenchymal stem cells of aplastic anemia patients and normal people.
METHODS: we collected bone marrow samples from six aplastic anemia patients and six normal persons. Ficoll stick wall method was used to isolate and culture bone marrow mononuclear cells that began to passage at 70%-80% confluence. The 3rd generation of mesenchymal stem cells were detected using flow cytometry. Total RNA was extracted to synthesis and design primer sequence, analysis standard curve, amplification curve, dissolved curve, to be compared with NaCan each gene genetic differences. Fluorescent quantitative PCR detection was employed for GATA-1, GATA-2, PPAR-γ, SCF gene in bone marrow mesenchymal stem cells of aplastic anemia patients and normal people.
RESULTS AND CONCLUSION: Expression of GATA-2 and SCF gene was lower (P=0.012, 0.039) and PPAR-γ expression was higher (P=0.035) in the aplastic anemia patients as compared with normal persons. There was no difference in GATA-1 expression. The findings indicated that abnormal expression of GATA-2 and SCF gene could affect macro-control of bone marrow hematopoietic microenvironment, and abnormal expression of PPAR-γ could explain why bone marrow adipogenesis easily formed in aplastic anemia patients.