Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3447-3450.doi: 10.3969/j.issn.1673-8225.2011.19.006

Previous Articles     Next Articles

A preliminary study of the number and identification of mesenchymal stem cells in spinal surgery bleeding

Ye Bin1, Qin Jian-pu2, Cai Yu-qiang2, Zhang Jun2, Chen Lin2, Liao Wen-bo2   

  1. 1Department of Orthopedics, Puren Hospital of Wuhan, Wuhan  430081, Hubei Province, China
    2Department of Orthopedics, Affiliated Hospital of Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Received:2010-11-27 Revised:2011-03-13 Online:2011-05-07 Published:2011-05-07
  • Contact: Liao Wen-bo, Doctor, Chief physician, Master’s supervisor, Department of Orthopedics, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China wenbo900@sina.com
  • About author:Ye Bin★, Master, Department of Orthopedics, Puren Hospital of Wuhan, Wuhan 430081, Hubei Province, China yebin5115@163.com
  • Supported by:

    Guizhou Provincial Education Committee, No. C-355*

PDF

327

Abstract:

BACKGROUND: A great amount of red bone marrow in the cancellous bone losses during orthopedic surgery.
OBJECTIVE: To explore the feasibility to isolate bone marrow mesenchymal stem cells (BMSCs) during spinal surgery bleeding and to investigate the relationship of the number of BMMSCs with age and time.
METHODS: Three cases of degenerative lumbar instability and 16 cases of lumbar burst fractures were selected to undergo posterior pedicle screw fixation, including 4 cases of 20-30 years, 5 of 30-40 years, 5 of 40-50 years, 2 of 50-60 years, 1 of 64 years, 1 of 65 years, and 1 of 67 years. 5 mL of vertebra blood was collected from 4 cases within 0-5 minutes after the awl for pierced vertebra was pulled out, and 5 mL of vertebral blood from other 15 cases was collected within 0-5, 5-10 and 10-15 minutes after the awl was pulled out, respectively. Mononuclear cells were isolated and incubated into 6-well plates, and then cultured for 3 days. The medium was renewed completely to remove unadherent cells. Hematoxylin-eosin staining was applied for cell counting. RESULTS AND CONCLUSION: The membrane mark showed that CD44 and CD29 were positive while the CD34 and CD14 were negative. The number of BMSCs isolated within 0-5 minutes was 262.47±172.20, and fewer or no BMSCs were found within 5-10 or 10-15 minutes. The number of BMMSCs from vertebra bleeding of 0-5 minutes had a decreased tendency with aging.

CLC Number: