Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (7): 1241-1244.doi: 10.3969/j.issn.1673-8225.2011.07.022

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Influence of Esculin and Digitalisglycosides Eye Drops on the proliferation and differentiation fibroblasts from pterygium

Zhang Li-gui1, Liu Hai-xia2, Qu Yan-gang3   

  1. 1Hubin Ophthalmological Hospital, Binzhou  256600, Shandong Province, China
    2Infomation Science and Engineering College, Ocean University of China, Qingdao  266100, Shandong Province, China
    3Department of Pathology, Central Hospital of Enshi, Enshi  445000, Hubei Province, China
  • Received:2010-08-04 Revised:2010-12-14 Online:2011-02-12 Published:2011-02-12
  • Contact: Zhang Li-gui, Doctor, Associate chief physician, Associate professor, Hubin Ophthalmological Hospital, Binzhou 256600, Shandong Province, China Liguizz@163.com
  • About author:Zhang Li-gui☆, Doctor, Associate chief physician, Associate professor, Hubin Ophthalmological Hospital, Binzhou 256600, Shandong Province, China Liguizz@163.com

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Abstract:

BACKGROUND: Studies have confirmed Esculin and Digitalis glycosides Eye Drops can improve tissue blood supply, while it remains unclear whether they accelerate the growth of pterygium in curing macular denaturation.
OBJECTIVE: To observe the effect of Esculin and Digitalis Glycosides Eye Drops on the proliferation of the fibroblasts of pterygium. 
METHODS: A total of 26 patients with pterygium in double eyes and macular denaturation in one eye were selected, and treated with Esculin and Digitalisglycosides Eye Drops. They were followed up for 3, 6, 12, 18 and 24 months. Images of pterygium were collected to observe the areas changing of prerygium. The pterygium in 8 patients was operated, and the fibroblasts from the removed tissues were cultured. Matrix metalloproteinase (MMP)-1, 3, 9 were determined by ELISA method and MMP-3 mRNA was detected by real-time fluorescent quantitative PCR method. In addition, the tissues from age-matched patients with primary pterygium and normal conjunctiva tissues from patients with cataract were used as control.
RESULT AND CONCLUSION: Following treatment with Esculin and Digitalisglycosides Eye Drops, the areas of the pterygium erosion cornea grew obviously rapidly (P  < 0.01). The contents of MMP-1 and MMP-3 of the fibroblasts of Esculin and Digitalisglycosides Eye Drops group were higher than that of control group and normal conjunctiva tissues (P  < 0.01), and the expression of MMP-3 mRNA was 52.7% greater than that of control group (P < 0.01). Results indicated that Esculin and Digitalisglycosides Eye Drops can promote the growth of pterygium, probably by promoting the secreting of MMP-1, 3 and promoting the expression of MMP-3 mRNA in fibroblasts.

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