Abstract:

BACKGROUND: Phospholipid polyunsaturated fatty acid (Phospholipid-PUFA) can inhibit the cell inflammatory response and immune function. The formation of hypertrophic scars has contact with inflammation, cellular immunity and cytokines closely. However, there are few studies addressing Phospholipid-PUFA on hypertrophic scars.
OBJECTIVE: To explore the inhibiting effect of phospholipid-PUFA on rabbit ear hypertrophic scar.
METHODS: Nine New Zealand rabbits were chosen to establish animal models of hypertrophic scars. Six round full-thickness wounds in sizes of 1 cm×1 cm were made in the ventral surface of each ear. Totally 108 wound were prepared, and formed 92 hypertrophic scars, with scar formation rate of 85%. Every 3 wounds in each front ear were treated by phospholipid-PUFA cream, those at the behind of right ear was treated by mult-acid mucopolysaccharide cream, once per day, the rest served as control. The wound healing was observed at 28, 42, 63 and 90 days after operation. The thickness of scars, collagen fiber and density of fibroblasts were observed under a microscope. The expression of collagen fiber was detected by immunohistochemistry.
RESULTS AND CONCLUSION: Compared with the control group, the volume of hypertrophic scar was minificated, thinner, the density of fibroblasts was diminished, and the expression of collagen fiber was decreased in the experimental groups, especially obviously in the phospholipid-PUFA group, suggesting phospholipid-PUFA can inhibit hypertrophic scar formation and reduce scar hyperplasia.