Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (7): 1232-1236.doi: 10.3969/j.issn.1673-8225.2011.07.020

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Effect of aerobic exercise on the expression of tuberous sclerosis complex-2 and insulin receptor substrate 1 serine phosphorylation of skeletal muscle in mice

Yang Feng-ying1, Niu Yan-mei1, Liu Yan-hui2, Chen Ni1, Wang Jia-zhong1, Fu Li1,2   

  1. 1Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin  300070, China
    2Department of Health and Exercise Science, Tianjin Institute of Physical Education, Tianjin  300381, China
  • Received:2010-09-06 Revised:2010-12-07 Online:2011-02-12 Published:2011-02-12
  • Contact: Fu Li, Doctor, Doctoral supervisor, Professor, Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin 300070, China; Department of Health and Exercise Science, Tianjin Institute of Physical Education, Tianjin 300381, China lifu@tijmu.edu.cn
  • About author:Yang Feng-ying☆, Studying for doctorate, Lecturer, Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin 300070, China nini@tijmu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30871213*; the Applied Basic Research and Leading Technology Projects, No. 09JCZDJC17400*

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Abstract:

BACKGROUND: Studies demonstrated that, expressions of insulin receptor substrate 1 (IRS1) serine phosphorylation and tuberous sclerosis complex-2 (TSC2) and abnormity of mammalian target of rapamycin (mTOR)/S6K1 signaling pathway play an important role in the agitation of insulin resistance.
OBJECTIVE: To study the effects of aerobic exercise on TSC2 and IRS1 serine phosphorylation activity of skeletal muscle in mice.
METHODS: C57BL/6 mice were randomly divided into the control group and exercise group. Mice in the exercise group were acclimatized to the motorized treadmill by running at the intensity of 75%VO2max, for 6 weeks. No intervention was performed in the control group. The expression and location of TSC2, IRS1, IRS1-Ser307 and IRS1-Ser636/639 in mouse skeletal muscle were measured by RT-PCR, Western blot, and immunofluorescence.
RESULTS AND CONCLUSION: The expression of TSC2 mRNA and protein of the exercise group were significantly increased compared with the control group (P < 0.05). There were no significant difference between two groups in the expression of IRS1 mRNA and protein. And the expression of pIRS1-Ser307 and pIRS1-Ser636/639 protein was significantly decreased in the exercise group compared with the control group (P < 0.05). The aerobic exercise probably agitate insulin pathway by enhancing the expression of TSC2 which attenuating the activity of IRS1-Ser307 and IRS1-Ser636/639 phosphorylation. 

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