Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (7): 1219-1223.doi: 10.3969/j.issn.1673-8225.2011.07.017

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Secondary repair effects of low-intensity pulsed ultrasound on class Ⅱ furcation involvement in Beagle dogs

He Ping1, Gao Xiang1, Song Jin-lin1, Deng Feng1, Zhao Chun-liang2, Wang Zhi-biao2, Li Na3     

  1. 1Department of Orthodontics, College of Stomatology, Chongqing Medical University, Chongqing  400015, China
    2Chongqing Key Laboratory of Ultrasound Medical Engineering, Chongqing  400016, China
    3Department of Stomotology of the Affiliated Hospital of North Sichuan Medical College, Nanchong  637000, Sichuan Province, China
  • Received:2010-11-29 Revised:2011-01-13 Online:2011-02-12 Published:2011-02-12
  • Contact: Song Jin-lin, Doctor, Chief physician, Professor, Master’s supervisor, Department of Orthodontics, College of Stomatology, Chongqing Medical University, Chongqing 400015, China soongjl@163.com
  • About author:He Ping★, Studying for master’s degree, Attending physician, Department of Orthodontics, College of Stomatology, Chongqing Medical University, Chongqing 400015, China hping78@gmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30870754*; the Major High-tech Industry Development Projects in Industrial Technology of Chongqing  Municipal Development and Reform Commission, Chongqing Development and Reform Technology  No. [2007]1110*; Research Projects of Chongqing Municipal Health Bureau, No. 2008-2-254*

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Abstract:

BACKGROUND: There were many reports on low-intensity pulsed ultrasound (LIPUS) to promote fracture healing and increase bone density, there were not literature involved in tissue repair effects of LIPUS on class Ⅱ furcation involvement.
OBJECTIVE: To investigate the tissue repair effects of LIPUS treatment on class Ⅱ furcation involvement.
METHOD: Beagle dogs’ class Ⅱ furcation involvement models were established at buccal region of the dogs’ fourth premolar of bilateral mandibular and randomly divided into ultrasonic treatment group (UT group) and control group (C group) and set notch at the gingival side of buccal root surface as the reference point. Then, Beagle dogs were fed with high glucose for 8 weeks. Scaling and planning of the root surface was applied on UT group and 1 week later take LIPUS (90 mW/cm2, 1.5 MHz, 200 μs,   1 kHz) to irradiate UT group by 20 minutes per day, while scaling and planning of the root surface and sham irradiation were applied on C group. After 6 weeks, decalcified tissue sections of hematoxylin-eosin staining and Masson staining were used to measure and analysis the furcation tissues.
RESULTS AND CONCLUSION: In the UT group, new alveolar bone of furcation area grew along the root surface and gingival epithelium appeared; in the C group, it mainly changed by connective tissue growth at furcation area as well as a large number of gingival epithelium and a small amount of new alveolar bone. The color of new alveolar bone collagen in the UT group was between red and blue, the color of new alveolar bone collagen in the C group showed blue, which meant the new alveolar bone collagen in the UT group was more mature than it in the C group. The new volumes of alveolar bone, cementum and periodontal ligament in the UT group were more than those in the C group (P < 0.05). So 90 mW/cm2 LIPUS can promote new bone collagen maturation and remodeling at furcation area.

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