Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (7): 1215-1218.doi: 10.3969/j.issn.1673-8225.2011.07.016

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Establishment of spinal cord transection injury models in rats

Meng Bu-liang1, Ba Ying-chun1, Song Shi-na2, Chen Shan-shan2, Li Li-yan2, Wang Ting-hua2   

  1. 1Department of Anatomy, 2Institute of Neuroscience, Kunming Medical College, Kunming  650031, Yunnan Province, China
  • Received:2010-09-02 Revised:2010-12-03 Online:2011-02-12 Published:2011-02-12
  • Contact: Wang Ting-hua, Doctor, Professor, Institute of Neuroscience, Kunming Medical College, Kunming 650031, Yunnan Province, China
  • About author:Meng Bu-liang☆, Studying for doctorate, Lecturer, Department of Anatomy, Kunming Medical College, Kunming 650031, Yunnan Province, China mbloso@126.com

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Abstract:

BACKGROUND: The spinal cord injury (SCI) models reported in literature were short of determinacy and reliability.
OBJECTIVE: To establish an accurate and reliable rat spinal cord transection injury models.
METHODS: Rats were divided into model and sham-surgery groups at random. Model group prepared for T10 transection injury models; vertebral canal and spinal dura mater were exposed and sutured in the sham-surgery group. The motor function of hind limbs was evaluated by Basso, Beattie and Bresnahan (BBB) scores; the integrality of the nervous pathways was detected by somato-sensory evoked potential (SEP) and motor evoked potential (MEP), in addition, the pathologic and gross pathology of spinal cord were performed at 1, 3, 5 and 7 days after modeling.
RESULTS AND CONCLUSION: Compared with the sham-surgery group, the BBB scores of the model group were decreased at 1, 3, 5 and 7 days after modeling (P < 0.01). The SEP and MEP were not evoked out in the model group. The spinal cords were fully transected in the model group, which was intact in the sham-surgery group. that of. The result suggested that a spinal cord transection injury model is established successfully.

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