Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (7): 1195-1198.doi: 10.3969/j.issn.1673-8225.2011.07.012

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Transforming growth factor beta 1 on proliferation and migration of adventitia fibroblasts in vitro

Ma Shao-jun, Cai Wen-wei, Sheng Jing   

  1. Department of Geriatrics, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai  200011, China
  • Received:2010-08-19 Revised:2010-09-05 Online:2011-02-12 Published:2011-02-12
  • Contact: Sheng Jing, Master, Chief physician, Department of Geriatrics, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China shengjing60@163. com
  • About author:Ma Shao-jun★, Master, Attending physician, Department of Geriatrics, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China mashj@163.com
  • Supported by:

    the Science and Technology Foundation of Shanghai Jiao Tong University School of Medicine, No. 06XJ21002*

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Abstract:

BACKGROUND: Previous studies demonstrated that adventitia fibroblasts exhibit important role in the hyperplasia of newly born endomembrane after blood vessel injury. Transforming growth factor β1 (TGF-β1) can stimulate many signways after blood vessel injury. However, the effect of TGF-β1 on proliferation and migration of adventitia fibroblasts in vitro remains unclear.
OBJECTIVE: To observe the effect of TGF-β1 on proliferation and migration of adventitia fibroblasts in vitro.
METHODS: Fibroblasts from rat thoracic aorta were in vitro cultured and stimulated by TGF-β1 with different concentrations (0, 3, 5, 10 and 15 μg/L) for different hours (0, 2, 12, 24, 48, and 72 hours). MTT assay were applied to assess cellular proliferation. Migration assay was performed by transwell chambers.
RESULTS AND CONCLUSION: TGF-β1 could potentiate the proliferation of adventitia fibroblasts, and the effects were increased with the increasing of TGF-β1 concentrations and TGF-β1 inducing times (P < 0.05), especially stronger in the group treated by 10 μg/L TGF-β1 for 48 hours (P < 0.01). The migration of adventitia fibroblasts were increased with the increasing of TGF-β1 concentrations in a dose-dependent fashion.

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