Abstract:

BACKGROUND: Except osteoblast and osteoclast that can lead to osteoporosis, D-galactose also can result in bone loss in male rats. 
OBJECTIVE: To observe the effect of D-galactose on bone tissue in vivo and osteoblast cultivated in vitro.
METHODS: Male SD rats were divided into control and D-galactose groups. Physiological saline was daily injected into rats in the control group, and 100 mg/kg D-galactose (5%) was administrated for 3 successive months. The left proximal tibia and fourth lumber were harvested for measurement of bone dynamic and static parameters using picture analyzing instrument. Osteoblast was in vitro cultured with different dose D-galactose and prostaglandin, the proliferation of osteoblasts was measured by MTT, and the alkaline phosphatase activity of osteoblast was detected by PNPP way.
RESULTS AND CONCLUSION: Compared with the control group, trabecula area, thichness, and number were decreased, but trabecula separation was increased in the D-galactose group, suggesting that bone mass lose of D-galactose rats. Decreased bone mineralization rate and bone formation rate indicating bone conversion velocity was decreased. D-galactose can induce low conversion rate-induced bone loss, which is not by inhibiting osteoblast directly, but by other indirect ways.