Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (3): 450-452.doi: 10.3969/j.issn.1673-8225.2011.03.017

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Effects of nano-zirconium dioxide-toughened hydroxyapatite bioceramics on proliferation and differentiation of rabbit bone marrow stromal cells

Tang Yue-jun1, Wang Xin-ling2, Zhou Zhong-hua3, Lü Chun-tang3   

  1. 1Stomatology Hospital Affiliated to Suzhou Health and Technology College, Suzhou   215006, Jiangsu Province, China
    2Department of Stomatology, First Outpatient Clinic, General Logistics Department of Chinese PLA, Beijing   100842, China
    3Department of Stomatology, Changhai Hospital, Second Military Medical University of Chinese PLA, Shanghai   200433, China
  • Received:2010-07-29 Revised:2010-11-09 Online:2011-01-15 Published:2011-01-15
  • Contact: Lü Chun-tang, Doctoral supervisor, Department of Stomatology, Changhai Hospital, Second Military Medical University of Chinese PLA, Shanghai 200433, China
  • About author:Tang Yue-jun☆, Doctor, Attending physician, Lecturer, Stomatology Hospital Affiliated to Suzhou Health and Technology College, Suzhou 215006, Jiangsu Province, China yjtang@szhct.edu.cn
  • Supported by:

    a grant by Jiangsu Provincial Health Department, No. Z200710

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Abstract:

BACKGROUND: Research group has performed some preliminary studies pertaining to zirconia toughened nano-hydroxyapatite (HA-ZrO2) bioceramics, mainly in the biomechanical compatibility, chemical stability, and biocompatibility. Among them, in vitro cell culture experiments are controllable and repeatable, can well reflect the material's biocompatibility.
OBJECTIVE: To study the influence to the growth and differentiation of rabbit bone marrow stromal stem cells by HA-ZrO2 or HA materials.
METHODS: The rabbit bone marrow stromal stem cells were cultured in beccos modified eagle’s medium (DMEM) containing 20% fetal bovine serum, the subsequent cell passage was cultured in conditioned medium containing dexamethasone, beta-sodium glycerophosphate and ascorbic acid. The third passage osteoblasts were cultured in cell culture plate with materials at 1.0×108/L. The proliferation of all the cultured cells were observed at 1-10 days under inverted phase contrast microscope and the curve of cell-growth was plotted. The activity of alkaline phosphatase was detected. At 6 days of the culture, cells and composite materials were fixed with paraformaldehyde and observed under scanning electron microscope.
RESULTS AND CONCLUSION: MTT assay showed the growth curve of cells cultured in two materials had no significant difference. The combined culture of rabbit bone marrow stromal cells can maintain normal function of secreting alkaline phosphatase. Electron microscopy photographs also confirmed the cell adhesion on two kinds of materials. HA-ZrO2 and HA show no adverse effect on the growth and differentiation of osteoblasts, with excellent compatibility.

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