Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (3): 436-440.doi: 10.3969/j.issn.1673-8225.2011.03.014

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Morphological changes of injured spinal cord following combined treatment of glial cell line-derived neurotrophic factor microspheres with NogoA and ChABC microspheres

Zhang Yu, Song Yue-ming, Li Tao, Liu Li-ming, Zeng Jian-cheng   

  1. Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu  610041, Sichuan Province, China
  • Received:2010-07-22 Revised:2010-08-30 Online:2011-01-15 Published:2011-01-15
  • Contact: Song Yue-ming, Doctor, Professor, Doctoral supervisor, Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China sym_cd@yahoo.com.cn
  • About author:Zhang Yu☆, Doctor, Attending physician, Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China yuxli@cisco.com
  • Supported by:

    the National Natural Science Foundation of China, No.30471759

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Abstract:

BACKGROUND: The principle of promoting axonal regeneration is to improve the environment that inhibits axonal regeneration and to enhance the capacity of axonal growth, the main measures are the use of axonal growth inhibitory factor blockers and neurotrophic factors. Biodegradable microspheres loaded drugs is a method to provide local sustained release of drugs.
OBJECTIVE: To evaluate the effect of glial cell line-derived neurotrophic factor (GDNF), NogoA and ChABC microspheres on the regeneration and functional recovery of rats following spinal cord injury from a view of pathomorphological point.
METHODS: Adult SD rats were used to establish spinal cord transective injury models, and were randomly divided into 8 groups: normal control group; sham operation group; local saline group; GDNF group; GDNF microspheres group; NogoA microspheres group; ChABC microspheres group; ChABC, GDNF and NogoA microspheres group. In normal control group and sham operation group, there was no model established. Ten weeks after injury, tetramethylrhodamine dextran amine anterograde neuronal tracing, immunohistochemistry and image analysis of neurofilament protein 200, GFAP and growth associated protein 43 were performed to assess the neurological regeneration.
RESULTS AND CONCLUSION: ChABC, GDNF, NogoA microspheres can upgrade the expression of neurofilament protein 200, GFAP and growth associated protein 43 at spinal cord injury site, thus the regeneration and repair after spinal cord injury are obvious. ChABC, GDNF, NogoA microsphere is better than GDNF microspheres for the treatment of spinal cord injury. ChABC, GDNF, NogoA microspheres can promote the regeneration and repair of rats with transected spinal cord injury and is better than GDNF microspheres.

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