Abstract:

BACKGROUND: Fibrin glue as a biological carrier of antibiotics, has been widely used in clinical work, but experimental study on fibrin glue in combination with the fourth generation of fluoroquinolone broad-spectrum antibiotic gatifloxacin has been rarely reported.
OBJECTIVE: To evaluate the effects of fibrin tissue adhesive and gatifloxacin on preventing and treating local infection of soft tissues after wound and the safety of the local drug delivery system．
METHODS: Standard local infection models were established in New Zealand healthy white rabbits, which were grouped randomly into experiment group, control group and blank group. Fibrin tissue adhesive and gatifloxacin were implanted into the local infection site in experiment group, gatifloxacin injected in control group and normal saline injected in blank group. Venous bloods of rabbit ear were collected before the operation and at 3, 7, 14 days after the operation, leukocyte counts of whole blood were determined respectively. The plasma level of gatifloxacin in experiment group was determined. Muscular tissues of lateral incision in experiment group were dislodged at 3, 7, 14 days after the operation to determine the level of gatifloxacin and histological examination.
RESULTS AND CONCLUSION: The blank group were infected in two weeks and the incisions were red and edema after the operation; There were no incision pus in experiment group and control group. Leukocyte count at different postoperative time points in experiment group was lower than that in blank group (P < 0.01), the control group was significantly higher than experiment group at 7 and 14 days, lower than blank group (P < 0.01 or P < 0.05). The plasma level of gatifloxacin and the level of gatifloxacin of 1.5 cm muscular tissues were gradually reduced at 3, 7, 14 days (P < 0.05); Histological examination revealed there were a lot of inflammatory cells in the muscular tissues of blank group. There were less inflammatory cells in control groups at 3 and 7 days, and no inflammatory cells at 14 days. Less inflammatory cells were found in experiment group at 3 days and no at 7, 14 days. The fibrin tissue adhesive is one kind of good biological carriers in anti-infection drug delivery system, its combination with gatifloxacin can reduce blooding and reduce infection rate.