Abstract:

BACKGROUND: In primary study, we obtained protein interacting with spinal cord injury and regeneration related gene No 10 (SCIRR10), which involving thyrotropin releasing hormone receptor 2 (TRH R2). However, the interaction between SCIRR10 and TRH R2 has been validated.
OBJECTIVE: To explore whether SCIRR10 has effect on TRH R2 and to certify whether TRH R2 is special receptor of SCIRR10.
METHODS: Recombinant plasmid expressing SCIRR10-GFP fusion protein and TRH R2-RFP fusion protein were constructed respectively using RT-PCR. The supernatant of these COS7 cells transfected with SCIRR10-GFP was collected and then added into those COS7 cells transfected with TRH R2-RFP, and later cells was cultured for another 4 hours. Treated cells were washed, fixed with 40 g/L paraformaldehyde, mounted. The results were observed under a laser scanning confocal microscope.
RESULTS AND CONCLUSION: SCIRR10-GFP could be seen combined with TRH R2-RFP under fluorescence. No similar results could be found in the control group. The results of this experiment and primary studies demonstrated that, TRHR R2 is the receptor of SCIRR10. However, the interaction protein of SCIRR10 has its special ligand.