Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (2): 298-301.doi: 10.3969/j.issn.1673-8225.2011.02.026

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Effects of endothelial nitric oxide synthase gene transfection on neointima hyperplasia after vascular injury

Hu Ping, Sheng Jing, Cai Wen-wei, Lu Ping   

  1. Department of Geriatrics, the Ninth People’s Hospital, Shanghai Jiao Tong University Medical College, Shanghai  200011, China
  • Received:2010-08-10 Revised:2010-11-11 Online:2011-01-08 Published:2011-01-08
  • Contact: Sheng Jing, Master, Chief physician, Department of Geriatrics, the Ninth People’s Hospital, Shanghai Jiao Tong University Medical College, Shanghai 200011, China kcb202123@126. com
  • About author:Hu Ping★, Master, Attending physician, Department of Geriatrics, the Ninth People’s Hospital, Shanghai Jiao Tong University Medical College, Shanghai 200011, China kcb202123@sina. com
  • Supported by:

    the Special Foundation for Excellent Youth Scholars of Shanghai Universities, No. jdy07069

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Abstract:

BACKGROUND: Nitric oxide (NO) can inhibit the proliferation and migration of vascular smooth muscle cells (VSMCs) and endothelial nitric oxide synthase (eNOS) is an important factor of the synthesis of NO. However, the effects of eNOS gene transfection on neointimal hyperplasia after balloon injury in atherosclerosis rats remain unclear.
OBJECTIVE: To study the inhibition effects of eNOS gene transfection on neointimal hyperplasia after balloon injury in atherosclerosis rats.
METHODS: The common carotid catheter injury model in atherosclerosis rats was established. All animals were divided into blank control, AdCMV-lacz and AdCMV-eNOS groups. Immediately after angioplasty, AdCMV-eNOS or AdCMV-lacZ or PBS was transfected into the injured segment of the common carotid artery for local therapy. Two weeks later, the transfected vascular were obtained to detect expression of eNOS mRNA in cultured smooth muscle cells. And then intimal-media ratio and neointimal hyperplasia were measured at different time points.
RESULTS AND CONCLUSION: The transfected common carotid arteries were confirmed the mRNA expression of eNOS gene in cultured smooth muscle cells. At 1 and 3 months after transfection, the intimal-media ratio was significantly reduced in AdCMV- eNOS transfected group than PBS group or AdCMV-lacZ transfected group (P < 0.01). eNOS gene transfection might strongly inhibit neointima hyperplasia after balloon injury in atherosclerosis rats and reduce arterial restenosis.

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