Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (2): 281-285.doi: 10.3969/j.issn.1673-8225.2011.02.022

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Effects of basic fibroblast growth factor combined with sodium hyaluronate on endothelial cells in corneal preservation solution

Yang Yue1,2, Zhao Min1   

  1. 1Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
    2Department of Ophthalmology, Chongqing Fifth People’s Hospital, Chongqing  400062, China
  • Received:2010-07-10 Revised:2010-08-13 Online:2011-01-08 Published:2011-01-08
  • Contact: Zhao Min, Professor, Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China inzhao2002@yahoo.com.cn
  • About author:Yang Yue★, Master, Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Ophthalmology, Chongqing Fifth People’s Hospital, Chongqing 400062, China yangyue_20049@yahoo.cn
  • Supported by:

    the Science and Technology Foundation of Health Bureau of Chongqing City, No. 2008-2-114

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Abstract:

BACKGROUND: In our previous studies, corneal preservation solution was improved by adding basic fibroblast growth factor (bFGF) combined with sodium hyaluronate, which be confirmed has effect on maintain endothelial cell activity.
OBJECTIVE: To explore effects of bFGF and sodium hyaluronate in corneal preservation solution and effective concentrations, in addition, to evaluate the protect effect of these two materials on corneal endothelial cells.
METHODS: 6% glucosan, HEPES buffer solution and antibiotics were added into MEM and M-199 to prepared corneal preservation solution, additionally, bFGF and sodium hyaluronate with different concentrations were added. Simultaneously, control group without bFGF and sodium hyaluronate was established. Cornea of New-Zealand white rabbits preserved in each group were detected by slit-lamp examination, trypan blue-alizarin red staining, and scanning electron microscope at 3, 5, 7, 10 and 14 days after operation.
RESULTS AND CONCLUSION: The transparency, endothelial cell activity, microstructure, as well as ultrastructure of the improved corneal preservation solution group were obviously better than those of the other group. Compared among the experimental groups, the high concentration group was superior to the low concentration group. The findings demonstrated that bFGF and sodium hyaluronate in corneal preservation solution can protect corneal endothelial cells, and the effectiveness is related to concentration.

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