Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (2): 273-276.doi: 10.3969/j.issn.1673-8225.2011.02.020

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Effects of aerobic exercise on expression of tumor necrosis factor alpha and adiponectin in adipose tissues of insulin resistant mice  

Chen Ni1, Niu Yan-mei1, Su Zhao-peng2, Liu Yan-hui2, Li Hui-ge2, Wang Jia-zhong1, Fu Li1,2   

  1. 1Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin 300070, China
    2Department of Health and Sports Science, Tianjin University of Sport, Tianjin  300381, China
  • Received:2010-09-02 Revised:2010-12-12 Online:2011-01-08 Published:2011-01-08
  • Contact: Fu Li, Doctor, Doctoral supervisor, Professor, Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin 300070, China; Department of Health and Sports Science, Tianjin University of Sport, Tianjin 300381, China lifu@tijmu.edu.cn
  • About author:Chen Ni★, Master, Teaching assistant, Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin 300070, China chenni1006@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30871213; the Natural Science Foundation of Tianjin, No.09JCZDJC17400

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Abstract:

BACKGROUND: Research has shown that aerobic exercise can affect insulin sensitivity through regulate the expression of peroxisome proliferators activated receptor γ (PPARγ) and related adipokines. But its mechanism has few reported.
OBJECTIVE: To investigate the possible mechanisms of aerobic exercise improving insulin resistance, study the effect of aerobic exercise on insulin resistant mice by observing the changes of the mRNA and protein expression of PPARγ, tumor necrosis factor  (TNF-α) and adiponectin in adipose tissue of insulin resistance C57BL/6 mice.
METHODS: C57BL/6 mice were fed by high fat diet for 10 weeks to establish animal models of insulin resistance. Then the mice were randomly divided into quiet and exercise groups. The exercise group was acclimatized to the motorized treadmill by running 60 min per day at the intensity of 75% VO2max, for 6 weeks. The expression of PPARγ, adiponectin, TNF-α mRNA and protein in mouse adipose tissues of two groups were detected by RT-PCR and Western blot.
RESULTS AND CONCLUSION: There was no significantly change on the expression of PPARγ mRNA and protein between groups (P > 0.05), but exercise could obviously increased adiponectin expression (P < 0.01), decreased TNF-α expression (P < 0.05), and dramatically decreased serum triacylglycerol and free fatty acid levels (P < 0.05, P < 0.01). The findings demonstrated that, aerobic exercise indirectly regulates adipose tissue insulin sensitivity by regulating PPAR-γ related adipokines expression. Aerobic exercise could significantly enhance insulin sensitivity and attenuates insulin resistance in C57BL/6 mice.

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