Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (2): 265-268.doi: 10.3969/j.issn.1673-8225.2011.02.018

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Effect of platelet-derived growth factor beta on in vitro proliferation and differentiation of human periodontal ligament cells   

Lin Jing-guang, Qi Feng, Ge Yi-ming, Han Jie   

  1. Second Affiliated Hospital of Liaoning Medical University, Stomatology Hospital of Liaoning Medical University, Jinzhou  121000, Liaoning Province, China
  • Received:2010-07-14 Revised:2010-08-28 Online:2011-01-08 Published:2011-01-08
  • About author:Lin Jing-guang, Chief physician, Professor, Second Affiliated Hospital of Liaoning Medical University, Stomatology Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China 379311389@qq.com; linjg138@sohu.com
  • Supported by:

    the Science and Technology Research Program of Education Department of Liaoning Province, No. 2008339

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Abstract:

BACKGROUND: Periodontal supporting tissues destroy and attachment losses are the main reasons for tooth loss. Cytokine can promote periodontal tissue regeneration.
OBJECTIVE: To research the effect of platelet-derived growth factor-β (PDGF-β) in the human periodontal ligament cells proliferation.
METHODS: Human fibroblasts were cultured with tissue block method in vitro. The passaged cells were identified by Bosi protein, keratin immunohistochemistry for qualitative analysis. PDGF-β was added to induce periodontal ligament cells proliferation, and the absorbance value was measured by MTT method.
RESULTS AND CONCLUSION: The cell proliferation was accelerated after adding 10 μg/L PDGF-β factor, the absorbance value of the experimental group was greater than that of the control group (P < 0.05). The findings demonstrated that PDGF-β can promote the proliferation of human periodontal ligament cells.

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