Abstract:

BACKGROUND: Angiogenesis affects by growth factor, Radix Astragali and vascular endothelial growth factor (VEGF) have synergistic effect on angiogenesis, but its mechanism remains unclear.
OBJECTIVE: To observe the effects of Radix Astragali injection on the mechanism of in vitro angiogenesis by comparing with intervention in a single VEGF.
METHODS: Radix Astragali injection and VEGF was cultured with aortic endothelial cells. The promotion of Radix Astragali injection on in vitro angiogenesis was observed by tests of cell proliferation, cell migration and tube formation. The VEGF expression was determined by Western blot.
RESULTS AND CONCLUSION: Radix Astragali injection could promote rat aortic endothelial cell proliferation (P < 0.01), increase the number of migrated cells (P < 0.01), induce aortic endothelial cells increase the number of tube formation, and significantly increase VEGF expression (P < 0.01). The findings demonstrated that, Radix Astragali injection can promote endothelial cell proliferation, migration and tube formation and has a significant effect on promoting in vitro angiogenesis, which achieved by increasing VEGF expression.