Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (2): 228-231.doi: 10.3969/j.issn.1673-8225.2011.02.009

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Establishment of a rabbit anterior cruciate ligament reconstruction model 

Zhang Li1, Tian Jing1, Tang Yin-li2, Min Shao-xiong1   

  1. 1Center of Orthopaedics, 2Department of Digestion Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2010-08-09 Revised:2010-11-13 Online:2011-01-08 Published:2011-01-08
  • Contact: Tian Jing, Master, Professor, Center of Orthopaedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China tian_jing6723@ yahoo.com.cn
  • About author:Zhang Li☆, Doctor, Attending physician, Center of Orthopaedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China tension_zh@163. com
  • Supported by:

    the Foundation of Health Department of Guangdong Province, No. 2009A425

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Abstract:

BACKGROUND: The tendon-bone interface heals slowly in the anterior cruciate ligament reconstruction model prepared by traditional method and requires long modeling periods.
OBJECTIVE: To improve the strength and speed of tendon-bone healing and build up an animal model of anterior cruciate ligament reconstruction by injecting fibrin glue (FG) containing recombinant bone morphogenetic protein-2 (rhBMP-2) into the interface between the tendon graft and the bone tunnel.
METHODS: Healthy adult New Zealand rabbits were prepared anterior cruciate ligament reconstruction models using its semitendinosus tendons. The FG containing rhBMP-2 was applied to the part of the graft that went into the bone tunnel in the model group. The control group received either FG without rhBMP-2 or no FG. There was no operation in the normal group. Specimens were collected at 4, 8 weeks after surgery respectively for biomechanical test.
RESULTS AND CONCLUSION: The ultimate load and stiffness of the model group were greater than those of the control and FG groups at the individual 4- and 8-week time points (P < 0.01). These findings show that rhBMP-2 could improve the ultimate load to failure and stiffness in the tendon-bone interface after early ACL reconstruction. The modeling period of anterior cruciate ligament reconstruction is shortened by new methods.

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