Abstract:

BACKGROUND: Rat implanted CBRH-7919 hepatic cancer models are important tools for the experimental therapy and imageologic diagnosis. However, how to determine and verify the established models remains poorly understood.
OBJECTIVE: To study the imageologic and pathologic findings of implanted CBRH-7919 hepatic cancer model in Wistar rats.
METHODS: Concentrated cancerous suspension of CBRH-7919 cell lines were injected subcutaneouly into the nude mice scapular region. Then the tumor tissues were implanted into the liver of adult Wistar rats under direct vision. At 4 weeks after implantation, models were confirmed by color ultrasonograph and observed by imageologic and pathologic examination.
RESULTS AND CONCLUSION: Totally 37 nodes were found by ultrasonograph in 35 successful models, and additional 4 nodes were found by magnetic resonance imaging (MRI), with the diameter of the largest tumor was 8 mm, and the least was 3 mm. The tumor presented with hypointensity signal on T1WI and hyperintensity signal on T2WI and fat-suppression images. Digital subtraction angiography showed that all nodes had a large amount of blood supply which came from the liver arteries. The tumor cells are variable in sizes and shapes, disarranged and remarkable atypia with light microscopic observation. All findings demonstrated that, color ultrasonograph and MRI are effective and convenient to examine the model rats, but digital subtraction angiography can help us to find the blood supply of tumors. MRI and digital subtraction angiography examination can show pathological and biological behavior changes of tumor in rat implanted hepatic cancer models.