Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (2): 205-207.doi: 10.3969/j.issn.1673-8225.2011.02.004

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Fluctuation of total collagen, type Ⅰ and Ⅱ collagen protein contents in the knee joint of osteoporotic rats 

Zhao Liang-jun1, Peng Fen-fen2, Zhuang Wei2, Tang Xiao-zheng1   

  1. 1Department of Orthopaedics, 2Department of Nephrology, Jiangxi Provincial People’s Hospital, Nanchang  330006, Jiangxi Province, China
  • Received:2010-07-19 Revised:2010-08-25 Online:2011-01-08 Published:2011-01-08
  • Contact: Tang Xiao-zheng, Chief physician, Professor, Master’s supervisor, Department of Orthopaedics, Jiangxi Provincial People’s Hospital, Nanchang 330006, Jiangxi Province, China nanchang2006@yahoo.cn
  • About author:Zhao Liang-jun★, Studying for master’s degree, Physician, Department of Orthopaedics, Jiangxi Provincial People’s Hospital, Nanchang 330006, Jiangxi Province, China zhaoliangjun163@163.com

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Abstract:

BACKGROUND: Studies demonstrated that collagen protein plays an important role in osteocytes proliferation and differentiation, sclerotic formation and absorption, as well as matrix mineralization.
OBJECTIVE: To observe the fluctuation of total collagen, type Ⅰ and Ⅱ collagen protein contents in knee joint of osteoporotic rats.
METHODS: Totally 40 female Sprague Dawley rats were randomly assigned into the experimental and control groups. In the experimental group, rat knee joint cartilage and cruciate ligament were harvested at 16 weeks after bilateral ovariectomy. The protein contents of total, type Ⅰ and Ⅱ collagen were measured by micro-hydroxyproline assay and enzyme linked immunosorbent assay.
RESULTS AND CONCLUSION: Compared with the control group, the protein contents of total, type Ⅰ and　Ⅱ　collagen were notably decreased (P < 0.05), and the type Ⅰ/Ⅱ collagen ratio was also declined in the experimental group (P < 0.05). The results suggested that osteoporosis leads to fluctuations of total collagen, type Ⅰ, Ⅱ collagen protein contents and type I/II collagen ratio in knee joints, which closely relates to knee osteoarthritis.

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