Abstract:

BACKGROUND: Freeze-dried bone is a most commonly used bone substitute material in clinical practice. Incorporation of strontium into freeze-dried bone will promote the performance of the material in clinic.
OBJECTIVE: To observe the influence of solution concentration and soaking time on incorporation of strontium into frozen dried bone by soaking method and further investigate the releasing characteriatics of strontium element from the combined material.
METHODS: Strontium was incorporated into frozen dried bone with solution concentration varying from 5 to 40 mmol/L. At     10 mmol/L of strontium concentration, the incorporation rates at different soaking time of 1.5, 3, 6, 9 days were analyzed. Controlled release of strontium ion was evaluated in deionized water. ICP-OES plasma emission spectroscopy was used to quantify the elements.
RESULTS AND CONCLUSION: With the increase of solution concentration, incorporation of strontium into frozen dried bone increased between 2.0% and 5.1%. Within 9 days observed, incorporation of strontium also increased along with soaking time. At the initial period, there was a burst-release of strontium, then release of strontium got steady and release of strontium was always higher than calcium within 30 days. According to this study, higher concentration of strontium solution and longer soaking time can improve the quantity of strontium incorporated into frozen dried bone. Strontium-incorporated bone has the capability to release strontium ion for a long period.