Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (51): 9601-9604.doi: 10.3969/j.issn.1673-8225.2010.51.022

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Effect of human hepatic failure serum on function of hepatocytes cultured in vitro: A simulation experiment

Xue Kun1, Gao Sen 1,2, Pan Ming-xin1, Gao Yi1   

  1. 1 Second Hepatobiliary Division, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou   510282, Guangdong Province, China; 2 Second Department of General Surgery, Second People’s Hospital of Hefei, Hefei  230000, Anhui Province, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Pan Ming-xin, Doctor, Chief physician, Second Hepatobiliary Division, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China nfygaosen@sina.com
  • About author:Xue Kun, Attending physician, Second Hepatobiliary Division, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China Gao Sen★, Master, Physician, Second Hepatobiliary Division, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China; Second Department of General Surgery, Second People’s Hospital of Hefei, Hefei 230000, Anhui Province, China nfygaosen@sina.com
  • Supported by:

    the National High-Tech Research and Development Program of China (863 Program), No. 2006AA02A141*

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Abstract:

BACKGROUND: The key material of bioartificial liver is the hepatocytes, whose function directly affects the clinical results.
OBJECTIVE: To simulate a composite with similar biological effects with hepatic failure serum on the hepatocytes function cultured in vitro.
METHODS: The efficacy of the simulated serum liver failure, human liver failure serum and 10% fetal bovine serum on the cultured CL-1 cells was observed and compared, including cell morphology and cell viability, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase and glutathione secretion.
RESULTS AND CONCLUSION: The simulation liver failure and human liver failure serum had the same efficacy on CL-1 cell viability, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase and glutathione secretion, with no significant difference (P < 0.05), CL-2 cell growth was inhibited, and CL-1 cells had good function state in 10% fetal bovine serum. The simulated liver failure serum and human liver failure serum have the same biological effect on CL-1 cells.

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