Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (51): 9582-9586.doi: 10.3969/j.issn.1673-8225.2010.51.018

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Nano-bone composite of bone marrow stromal stem cells and nano-material scaffold in repairing rabbit femoral head necrosis

Mu Xiao-hong1, Zhao Zi-yi2, Xu Lin2, Wang Shuo-ren3, Zhu Ling-qun3, Chen Jiang2, Li Xiao-ping2   

  1. 1 Beijing University of Chinese Medicine, Beijing   100029, China; 2 Department of Orthopaedics, 3Central Laboratory, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing   100700, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Zhao Zi-yi, Doctor, Associate chief physician, Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China zhaoziyi@hotmail.com
  • About author:Mu Xiao-hong☆, Doctor, Associate chief physician, Beijing University of Chinese Medicine, Beijing 100029, China mxh__2004@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30600847*

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Abstract:

BACKGROUND: The chemical properties and physical properties of nano-hydroxyapatite collagen (nHAC), prepared by Department of Materials at Tsinghua University, for bone repair, are in line with environmental requirements in the human body, and nHAC has good biocompatibility.
OBJECTIVE: To evaluate the effectiveness of bone marrow mesenchymal stem cells (MSCs) and nHAC in osteonecrosis of the femoral head by means of nano-materials and tissue engineering technology.
METHODS: MSCs and nHAC were mixed to prepare a complex. Bilateral osteonecrosis of the femoral head models were established in 46 New Zealand rabbits and then were randomly divided into 3 groups. Sham-operation group received no treatment; nHAC group was implanted with nHAC; nHAC+MSCs group was implanted with the complex of nHAC+MSCs. Imageology and histology changes of femoral head were investigated at 4, 8, 12 weeks after operation respectively.
RESULTS AND CONCLUSION: X-ray examination showed that at 12 weeks after implantation, the femoral head collapse was found in sham-operation group; there was no obvious difference between implanting area and surrounding tissue after nHAC implantation, new bone trabeculae was around; in nHAC+MSCs group, the implanting area was similar to the surrounding tissue, and trabecular bone distributed. Histological detection showed that: at 4 weeks after operation, the sham-operation group presented no significant changes in defect area, while nHAC degradation and new bone formation were observed in nHAC group and nHAC+MSCs group; at 8 weeks after operation, the articular cartilage of sham-operation group was defected while osteogenesis and nHAC degradation were more obvious in nHAC group and nHAC+MSCs group; at 12 weeks after operation, there were collapse in some cases of sham operation group; At the same time, defect area was restored in nHAC group and nHAC+MSCs group, and trabecularism formed in nHAC+MSCs group. nHAC and nHAC+MSCs are superior to sham operation group in terms of the osteonecrosis of femoral head, especially nHAC+MSCs.

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