Abstract:

BACKGROUND: Chitosan scaffolds loading sustained-releasing transforming growth factor β microspheres can promote chondrocyte grow in vitro, and induce bone marrow mesenchymal stem cells differentiation into chondrocytes. Chitosan scaffolds loading sustained-releasing microspheres are expected to be a booming tissue engineered material for the treatment of cartilage defects. However, the biocompatibility is not disregardful for in vivo experiments.
OBJECTIVE: To prepare chitosan scaffolds loading sustained-releasing microspheres, and evaluate its biocompatibility in vivo and in vitro.
METHODS: By using hemolytic test, acute systemic toxicity test, intracutaneous stimulation test, heat source experiment and intramuscular implantation test, the biocompatibility of the self-made chitosan scaffolds loading sustained-releasing microspheres was comprehensively evaluated.
RESULTS AND CONCLUSION: The hemolytic rate of chitosan scaffolds was 1.6%, there was no significant destruction of red blood cells observed via the microscope; the acute toxicity was evaluated as non-toxicl; the intracutaneous primary stimulation scores and the primary stimulation index were both 0; in the heat source experiment, the body temperature raise to (0.17±   0.06) ℃; rats underwent intramuscular implantation all survived well without systemic infection, and newborn hair normally distributed at 4 weeks; Naked-eye observed that there were significantly increased peripheral vascular stents at 8 weeks, which well integrated with the surrounding muscle tissue, while other internal organs such as heart , liver, lung and kidney were ordinary. Along with time prolonged, infiltration of lymphocytes was gradually decreased, blood vessels and fibrous tissues were visible to grow into the scaffolds, wrapped fibrous tissues were gradually thinner, chitosan scaffolds were gradually degraded. Chitosan scaffold loading porous microspheres has excellent biocompatibility.