Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (51): 9567-9571.doi: 10.3969/j.issn.1673-8225.2010.51.015

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Biological behavior of human dental pulp cells on hydroxyapatite/tricalcium phosphate scaffold with different pore sizes

Peng Wei-wei1, Zhai Wan-yin2, Jiang Long1, Li Li-fen1, Chang Jiang2, Zhu Ya-qin1   

  1. 1 Department of General Dentistry, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai  200011, China; 2 Biomaterial and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai   200050, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Zhu Ya-qin, Chief physician, Professor, Doctoral supervisor, Department of General Dentistry, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China zyq1590@163.com
  • About author:Peng Wei-wei★, Studying for master’s degree, Physician, Department of General Dentistry, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China zyq1590@163.com
  • Supported by:

    the Scientific Research Foundation for the Returned Overseas Chinese Scholars, No. 2008-0890-09*; Science and Technology Commission of Shanghai Municipality, No. 08DZ2271100*; Shanghai Key Laboratory of Oral Medicine, No. 08JC1414500*; Key Project of Scientific Research Innovation by Shanghai Municipal Education Commission, No. 09ZZ116*

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Abstract:

BACKGROUND: Studies prove the feasibility of repairing dental defects using human dental pulp cells combined with hydroxyapatite/tricalcium phosphate (HA/TCP) scaffolds by means of tissue engineering methods. However, it is not clear that which pore size of scaffold can promote the growth and differentiation of dental pulp cells well.
OBJECTIVE: To study the biological behaviors, such as adherence, proliferation and differentiation of human dental pulp cells on the HA/TCP scaffold with different pore sizes.
METHODS: Human dental pulp cells were seeded into the HA/TCP scaffold with three kinds of pore sizes. Fluorescence microscope and scanning electronic microscope were used to observe the adherence and growth of cells to HA/TCP. Subsequently, the adherence and proliferation were assayed by adhesion rate and MTT tests, respectively. The complex of human dental pulp cells and HA/TCP scaffolds with different pore sizes were cultured with both growth medium and osteogenic medium, then the activity of alkaline phosphatase was determined at 4, 7 and 10 days.
RESULTS AND CONCLUSION: Human dental pulp cells adhered to the surface and the pores of HA/TCP scaffold successfully, and proliferated well. The highest rate of adhesion was observed in the group of the scaffold with pore size in the range of 100-300 μm. Results of MTT showed that 300-500 μm pore size group could promote cellular proliferation after 3 days. The alkaline phosphatase activity of human dental pulp cells, compound with the scaffolds with pore size of 100-300 μm and 300- 500 μm, was significantly higher than 500-700 μm group after 10 days incubation. The scaffold with the pore size in range of either 100-300 μm or 300-500 μm promoted the adhesion, proliferation and differentiation of human dental pulp cells better than the group of 500-700 µm.

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