Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (47): 8764-8768.doi: 10.3969/j.issn.1673-8225.2010.47.006

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Histological variation of heterogeneous biological bone screw applied in fracture model

Zhang Ying, Liu De-hua, Xia Yuan-jun, Wu Wen, Xie Hui-bin, Tan Xin-yu, Liu Han-hui   

  1. Department of Trauma-orthopedics, Orthopedics Hospital, Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou   510010, Guangdong Province, China
  • Online:2010-11-19 Published:2010-11-19
  • About author:Zhang Ying☆, Doctor, Chief physician, Department of Trauma-orthopedics, Orthopedics Hospital, Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China zhangying_doc@yahoo.com.cn
  • Supported by:

    the Science and Technology Project of Guangdong Province, No. 2008B060600040*

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Abstract:

BACKGROUND: In recent years, the fixation of articular fracture using absorbable screws is achieved in more and more cases, but mechanical properties are instable and early absorption results in fixation failure. Therefore, it is necessary to develop xenogeneic bone screws of stable performance and affordable cost to be an alternative of absorbable screw.
OBJECTIVE: To observe the histology variation of heterogeneous biological bone screws applying in rabbit fracture model.
METHODS: Healthy adult New Zealand rabbits were adopted to prepare left femur condylar fracture in animal models and then were divided into experiment group and control group, which were respectively fixed using heterogeneous biological bone screws and absorbable polylactic screws. At 2, 4, 6, 8, 12, 24 weeks postoperation, the screws and tissue around bone screw were observed on histology.
RESULTS AND CONCLUSION: Animals in both groups gradually restored the activities 1-2 days later, and the activities were normal at 10 days, local wound had no swelling, ulceration, exudation or necrosis, all wounds completely healed in 2 weeks. Pathological sections revealed that at 2-4 weeks, inflammatory cell infiltrated between biological bone screw and host bone; at 6-12 weeks, the number of inflammatory cells gradually reduced, fibrosis was significantly obvious than before, fibrous edges appeared some osteoclasts; at 24 weeks, fiber layer thickness reduced, the number of osteoclasts significantly increased, and bone bridge was visible with host bone. Inflammatory cells infiltrated on the surface of the absorbable screw channel at 2-4 weeks after surgery; at 6-8 weeks, cancellous bone inflammatory cell infiltration decreased, fibrosis was significant, and no infiltration of osteoclasts was observed; at 12-24 weeks, the thickness of fibrous connective tissue layer on surface of the screw channel became thicker, some osteoclasts infiltrated in the host cancellous bone. Biological bone screws could induce ossification to a certain extent, and no obvious immunological rejection is observed in this experiment.

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