Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (46): 8651-.doi: 10.3969/j.issn.1673-8225.2010.46.024

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Expression and localization of protein 4.1 family in rat cerebral cortex neural cells

Che Shu-qin1, Liu Su-fang1, Han Xue-fei2, Yan Wen-hai3, Zhao Tian-chun1, Duan Ping1, Xing Ying 1,2   

  1. 1Department of Physiology, 2Stem Cell Research Center, 3Department of Pathophysiology, Basic Medical College of Zhengzhou University, Zhengzhou  450052, Henan Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: Xing Ying, Doctor, Professor, Doctoral supervisor, Doctor, Professor, Stem Cell Research Center, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan Province, China XingY@zzu.edu.cn
  • About author:Che Shu-qin★, Master, Department of Physiology, Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan Province, China cheshuqin2009@126.com

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Abstract:

BACKGROUND: Studies have showed that the protein 4.1 expression types and splicing vary significantly in different tissues and cells. The different structures of different protein domains and cellular localization of protein 4.1 are intrinsically linked.
OBJECTIVE: To observe the expression of the mRNA, protein and the localization of 4.1R, 4.1N, 4.1G, and 4.1B in rat cortical neurons.
METHODS: The expression of the mRNA, protein and the localization of protein 4.1 families in new born Sprague Dawley rats was detected by RT-PCR, Western blot, immunocytochemical staining and laser-scanning confocal microscopy. The differences of expression and localization of localization of 4.1R, 4.1N, 4.1G, and 4.1B in cells were compared.  
RESULTS AND CONCLUSION: Both mRNA and protein of 4.1R, 4.1N, 4.1G, and 4.1B in rat cortical neurons were expressed in vitro. 4.1R mainly appeared in nerve cell membrane and cytoplasm, especially highly expressed in neurite extension or axon extension. 4.1G mainly emerged in cytoplasm of nerve cells, especially the protruding parts. 4.1N mainly located in cytoplasm and membrane processes. 4.1B mainly expressed in cell bodies and in parts of the cytoplasm and membrane. The results demonstrated that the four kinds of protein 4.1 families in rat cortical neurons were expressed in vitro with distinct intracellular locations.

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