Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (46): 8576-.doi: 10.3969/j.issn.1673-8225.2010.46.007

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Expression of transforming growth factor beta 1 mRNA in alveolar bone of rats with type 2 diabetes mellitus combined with periodontal disease

Qu Pan, Gao Xiu-qiu   

  1. Second Affiliated Hospital of Liaoning Medical University, Jinzhou  121000, Liaoning Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: Gao Xiu-qiu, Master’s supervisor, Professor, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China jzgaoxiuqiu1988@yahoo.com.cn
  • Supported by:

    the Foundation of Education Department of Liaoning Province, No. 2008418*

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Abstract:

BACKGROUND: Research showed that diabetes can increase the risk of periodontal disease, diabetes can cause the disorder of bone metabolism and lead to osteoporosis, but the specific mechanisms remain unclear.
OBJECTIVE: To explore the mechanism of type Ⅱ diabetes mellitus on promoting periodontal disease by measuring rat alveolar expression of transforming growth factor β1 mRNA (TGF-β1 mRNA) and apical bone mineral density.
METHODS: SD rats were prepared for type Ⅱ diabetes mellitus models by using high glucose and high fat diet, low-dose streptozotocin intraperitoneal injection and orthodontic ligation wire ligation. Expression of TGF-1 mRNA in alveolar bone was detected by RT-PCR, and the alveolar bone was performed bone mineral density measurement and pathological observation.
RESULTS AND CONCLUSION: Compared with other groups, the expression of TGF-1 mRNA was greatest, and the bone mineral density was smallest in the type 2 diabetes mellitus combined with periodontal disease group (P < 0.01). Type Ⅱ diabetic rats with periodontal disease tended to alveolar bone osteoporosis. The results suggest that type Ⅱ diabetes mellitus possibly promote alveolar bone osteoporosis through over-expression of TGF-β1, and type Ⅱ diabetes mellitus can also pass the transforming growth factor-β1 over-expression to promote periodontal disease.

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