Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (41): 7784-7790.doi: 10.3969/j.issn.1673-8225.2010.41.044

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Effects of basic fibroblast growth factor on expressions of collagen and fibronectin in normal skin and hypertrophic scar fibroblasts

Song Rui 1, Bian Hui-ning2, Lai Wen2, Chen De-hua2, Zhao Ke-sen1   

  1. 1 Guangdong Key Laboratory of Shock and Microcirculation Research, Department of Pathophysiology, Southern Medical University, Guangzhou  510515, Guangdong Province, China; 2 Department of Burns, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou  510080, Guangdong Province, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Professor, Doctoral supervisor, Guangdong Key Laboratory of Shock and Microcirculation Research, Department of Pathophysiology, Southern Medical University, Guangzhou 510515, Guangdong Province, China zhaoks1937@yahoo.com
  • About author:Song Rui☆, Doctor, Guangdong Key Laboratory of Shock and Microcirculation Research, Department of Pathophysiology, Southern Medical University, Guangzhou 510515, Guangdong Province, China songrui110@126.com Bian Hui-ning, Associate chief physician, Department of Burns, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China Song Rui and Bian Hui-ning contributed equally to this paper.
  • Supported by:

     the National Natural Science Foundation of China, No. 30672179*; the Natural Science Foundation of Guangdong Province, No. 04000570*

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Abstract:

BACKGROUND: Basic fibroblast growth factor (bFGF) can promote production of collagen, fibronectin and matrix enzyme in healing wounds. However, dysregulation of this process, such as the abnormal coordination of cell proliferation, extracellular matrix and neovascularization formation, or remodeling of the wound matrix will lead to excess accumulation of scar tissues.
OBJECTIVE: To investigate effects of bFGF on normal skin wound healing and hypertrophic scar formation.
METHODS: Normal and hypertrophic scar fibroblasts from tissue biopsies from 5 patients who underwent plastic surgery for repairing hypertrophic scars were isolated and cultured. The expressions of collagen, fibronectin and protein synthesis were detected by RT-PCR and ELISA. The mitochondrial membrane potential changes were measured using JC-1 staining and flow cytometry. Simultaneously, adenosine triphosphate (ATP) levels were determined by chemiluminescence method. The effects of bFGF on these indexes of normal and hypertrophic scar fibroblasts were observed. 
RESULTS AND CONCLUSION: Hypertrophic scar fibroblasts become slower after being exposed to bFGF, which selectively inhibited type Ⅰ collagen production in hypertrophic scar fibroblasts (P < 0.05). Although bFGF inhibited type Ⅰ collagen production, it had no effect on type Ⅲ collagen expression in both normal and hypertrophic scar fibroblasts. However, fibronectin expression in the normal fibroblasts was up-regulated after bFGF treatment (P < 0.05). In addition, the mitochondrial membrane potential tended to depolarization, although no statistical difference, in hypertrophic scar fibroblasts treated with bFGF (10 or 100 μg/L). bFGF treatment increased the cellular ATP levels in the normal fibroblasts, while there were no significant alterations in the hypertrophic scar fibroblasts over a treatment of bFGF (10 or 100 μg/L, P < 0.05). The results suggest that there are differential effects and mechanisms on the skin fibroblasts with bFGF treatment in normal wound healing and hypertrophic scar formation.

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