Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (41): 7665-7670.doi: 10.3969/j.issn.1673-8225.2010.41.016

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Dynamic changes of inflammatory cells infiltration and related gene expression after limb ischemia in nude mice

Li Da-yong1, Zheng Qiao-nan1, Yuan Ming-dian1, Chen Wen-na2, Gu Feng3, Lü Yan-wei1   

  1. 1 Department of Vascular Surgery, Affiliated Hospital of Liaoning Traditional Chinese Medical University, Shenyang, 110032, Liaoning Province, China; 2 Teaching and Experiment Center of Liaoning Traditional Chinese Medical University, Shenyang  110032, Liaoning Province, China; 3 Department of Foundation of Traditional Chinese Medicine, Liaoning Traditional Chinese Medical University, Shenyang  110032, Liaoning Province, China　
  • Online:2010-10-08 Published:2010-10-08
  • About author:Li Da-yong☆, Doctor, Associate professor, Department of Vascular Surgery, Affiliated Hospital of Liaoning Traditional Chinese Medical University, Shenyang, 110032, Liaoning Province, China sylidy@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30600843*; National Key Technology Research and Development Program of China during the Eleventh-five Period, No. 2008BAI53B012*; Startup Foundation for Doctors of Science and Technology Commission of Liaoning Province, No. 20061030*

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Abstract:

BACKGROUND: Research has shown that compensatory angiogenesis may occur after ischemia, but the relation between the changes of the local inflammatory response and angiogenesis after ischemia has rarely reported.
OBJECTIVE: To discuss the dynamic changes of the inflammatory cells infiltration and related gene expression in muscle after limb ischemia.
METHODS: Femoral artery ligation method was used to establish nude mice right hind limb ischemia model. The ischemic changes of limb ischemia were observed at 3 days, 1, 2, 3 and 4 weeks after limb ischemia. Hematoxylin-eosin staining, CD34 and CD68 immunohistochemical staining were used to observe ischemic morphological changes, the microvascular count (MVC) and macrophages infiltration in muscle tissue respectively. Western blot and reverse transcription polymerase chain reaction were used to detect changes of nuclear factor-kappa B (NF-κB), monocyte chemotactic protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) in ischemic muscle.
RESULTS AND CONCLUSION: Limb ischemic state of nude mice was the most serious at 1-2 weeks after ischemia. After limb ischemia, muscle fibers shrink and deformation, and the expression of MCP-1 and VEGF were greatest (P < 0.01). MVC was the highest at 2 weeks after limb ischemia (P < 0.01). There were a large number of inflammatory cell infiltrations at 3 days-2 weeks, and parts of nude mice had ischemic gangrene, especially severe at 2 weeks, and the expression of NF-κB was enhanced    (P < 0.01). NF-κB and MCP-1 gene-mediated inflammatory response in limb ischemia was the compensatory changes, which stimulates VEGF gene expression and short-term process of angiogenesis. But the number of capillary formation is limited, thus, it was not enough to compensate the state of limb ischemic gangrene.

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