Abstract:

BACKGROUND: Glycolytic system plays an important role in the consumption and utilization of energy in movement. Various training subjects lead to different adaptive changes of bodies.
OBJECTIVE: To explore the effects of different training intensities on phosphofructokinse (PFK), hexokinase (HK) and pyruvate kinase (PK) activities of rat skeletal muscle.
METHODS: According to BEDFORD TG standards, anaerobic exercise, aerobic exercise, aerobic and anaerobic cross-training motion models were established using treadmill running with different intensities. At the same time, normal animals served as control group. All the animals were killed immediately after exercise training. The activity of PFK, HK and PK were determined by enzyme-coupled assay.
RESULTS AND CONCLUSION: After treadmill training, the PFK activity in the anaerobic exercise group was increased (P < 0.05). To the cross-training group, it increased after 6 weeks’ training (P < 0.05), however, the activity of PFK was decreased after 2 and 4 weeks’ aerobic exercise training (P < 0.05). HK activity was increased after 4 and 6 weeks’ training (P < 0.05), which was the highest in the anaerobic exercise group (P < 0.05). Compared with the cross-training group, HK activity in the aerobic exercise group was lower (P < 0.05). The activity of PK was decreased in 2-week and 4-week anaerobic exercise group than that of the control group (P < 0.05). The present findings demonstrated that, the activity of rate-limiting enzyme in glycolysis was affected not only by exercise modes, but also by training periods. Long-time training, especially including high-speed anaerobic training, can protect the activity of rate-limiting enzyme in glycolysis.