Abstract:

BACKGROUND: It is difficult to prepare complete and large piece of goat acellular dermal matrix using liquid nitrogen method due to breakability of dermal matrix during rewarming period. 
OBJECTIVE: To explore a better method for goat acellular dermal matrix fabrication.
METHODS: In the course of freezing goat skin, a -80℃ refrigerator was used as a substitute for liquid nitrogen. Cell components of goat skin were removed by freezing and thawing repeatedly, and then cell fragments were washed off using a zwitterionic with ultrasonic machine. The cell fragments and structure were tested. 
RESULTS AND CONCLUSION: By strict tests, collagen structure of the heterogeneous dermal matrix fabricated here was integrity and no cell fragment was found. The biomechanical characteristics were similar with acellular dermal matrix fabricated by routine repeated frozen method. Compared with routine repeated frozen method, the acellular degree and biological properties of goat acellular dermal matrix prepared by improved method had no significant differences, but the integrity of latter was eminent.