Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (41): 7621-7625.doi: 10.3969/j.issn.1673-8225.2010.41.006

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Effects of ghrelin on the proliferation and differentiation of human osteoblasts

Liao Le-le, Wang Wan-chun   

  1. Department of Orthopaedics, the Second Xiangya Hospital of Central South University, Changsha  410011, Hunan Province, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Wang Wan-chun, Doctor, Professor, Department of Orthopaedics, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China wwc1962@yahoo.com.cn
  • About author:Liao Le-le☆, Doctor, Physician, Department of Orthopaedics, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China L820207@163.com

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Abstract:

BACKGROUND: Ghrelin has been reported to have a role in the regulation of bone metabolism on rats, but whether it has the effects on the proliferation and differentiation of human osteoblasts remains unclear.
OBJECTIVE: To investigate the effects of ghrelin on the proliferation and differentiation of human osteoblasts.
METHODS: Human osteoblasts were separated from normal human cancellous bone in anterior superior spine, and cultured in vitro. The expression of GHS-R1a in human osteoblasts was verified by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Human osteoblasts proliferation was assessed by measuring [3H] thymidine (2Ci/ml) incorporation into trichloroacetic acid (TCA)-insoluble material. The differentiations of human osteoblasts were evaluated by RIA and ELISA. The expression of Runx2 in human osteoblasts was detected by Western blot.
RESULTS AND CONCLUTION: The mRNA and protein of GHS-R1a were expressed in human osteoblasts. Ghrelin promoted the proliferation of human osteoblasts in a dose-dependent manner (P < 0.01). Ghrelin increased the activity of alkaline phosphatase (P < 0.01) and the secretion of osteocalcin (P < 0.01) in human osteoblasts. But the protein expression levels of Runx2 in human osteoblasts were no significantly changed by the intervention of ghrelin. The findings demonstrated that ghrelin can efficiently promote the proliferation and differentiation of human osteoblasts.

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