Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (41): 7601-7604.doi: 10.3969/j.issn.1673-8225.2010.41.001

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Effect of calcitonin on beta-catenin and secreted frizzled-related protein 1 in articular cartilages

Zhang Yin-na1, Wang Wen-ya1, Zhang Liu2, Hu Hong-yu2, Li Bin2, Wang Zhe-yan1   

  1. 1 Department of Pathology, North China Coal Medical College, Tangshan  063000, Hebei Province, China; 2 Department of Orthopedic Surgery, Affiliated Hospital of North China Coal Medical College, Tangshan  063000, Hebei Province, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Wang Wen-ya, Professor, Department of Pathology, North China Coal Medical College, Tangshan 063000, Hebei Province, China Wangwenya-3@126.com
  • About author:Zhang Yin-na★, Master, Department of Pathology, North China Coal Medical College, Tangshan 063000, Hebei Province, China chengt2080@yahoo.com.cn
  • Supported by:

    the Science and Technology Innovation Leading Talent Fund Project of Hebei Province, No. 06547008D-8*

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Abstract:

BACKGROUND: Researches have reported the protection function of calcitonin for chondrocytes via preventing bone resorption, but the mechanism remains unclear.
OBJECTIVE: To assess whether calcitonin exerts a protective action through the Wnt signal pathway in chondrocytes.
METHODS: The chondrocytes were separated from Japanese male rabbits and randomly divided into the control, interleukin 1 β and calcitonin groups. In the later two groups, interleukin 1 β (1×10-5 g/L) was added at 2 days after passage to simulate osteoarthritis, and then DMEM containing calcitonin was used to culture the cells in the calcitonin group at 4 days after passage. The expression of β-catenin protein was detected by immunocytochemistry, and the mRNA of β-catenin and secreted frizzled-related protein 1 (sFRP-1) were determined by real-time PCR. 
RESULTS AND CONCLUSION: The expression of β-catenin and its mRNA in the calcitonin group was obviously lower than that of the interleukin 1 β group (P < 0.05). Compared with the control group, the expression of β-catenin and its mRNA in the interleukin 1 β group appeared to be greater (P < 0.05). The expression of sFRP-1 mRNA was notably higher in the calcitonin group than that of the interleukin 1 β group (P < 0.05), which was lower than the control group (P < 0.05). The results demonstrated that calcitonin can inhibit the further damage of articular cartilage, which may play its protective role via Wnt/β-catenin signal pathway.

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