Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (38): 7119-7123.doi: 10.3969/j.issn.1673-8225.2010.38.022

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Preparation and loading characteristics of cholesterol-modified pullulan conjugate nanoparticles

Bai Yong-gang1, Zhang Ming-ming1, Li Lei 1, Zhang Tong1, Liu Ling-rong 1, Zhang Qi-qing 1,2   

  1. 1 Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin   300192, China; 2 Research Center of Biomedical Engineering, Xiamen University, Xiamen   361005, Fujian Province, China　
  • Online:2010-09-17 Published:2010-09-17
  • Contact: Zhang Qi-qing, Doctor, Professor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, China; Research Center of Biomedical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China zhangqiq@xmu.edu.cn
  • About author:Bai Yong-gang★, Master, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, China baiyonggang19830@163.com
  • Supported by:

    The National Key Scientific Program - Nanoscience and Nanotechnology, No. 2006CB933300*; Doctoral Station Foundation, No. 20091106120051*

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Abstract:

BACKGROUND: Hydrophobically modified polysaccharides can self-assemble into micelle in water, are used as a carrier of hydrophobic micromolecule drugs and bioactive macromolecules (polypeptide, protein and gene), and have potential application values in biotechnology and medicine.
OBJECTIVE: To comprehensively investigate the influence of cholesterol-based degree of substitution and relative molecular weight of pullulan polysaccharides on the properties of modified pullulan nanoparticles.
METHODS: Various cholesterol-bearing pullulans with different molecular weights of parent pullulan and degrees of substitution of cholesteryl moiety were synthesized. The resulting cholesteryl-pullulan modified polysaccharides were self-assembled in water into nanoparticles. The influence of cholesterol-based degree of substitution and relative molecular weight of polysaccharides on the properties of nanoparticles was observed. With doxorubicin as model drug, the encapsulating and in vitro release behavior on cholesteryl-pullulan modified polysaccharide nanoparticles were investigated.
RESULTS AND CONCLUSION: With the degree of substitution and molecular weights increased, the stability of the nanoparticles enhanced, critical micelle concentration decreased. Loading capacity and encapsulation efficiency of the nanoparticles increased with increasing of molecular weights of the parent pullulan and decreasing of cholesterol degree of substitution in the same feed ratio. Higher feed ratio could lead to higher loading capacity and lower encapsulation efficiency under the same degree of substitution of cholesteryl moiety and molecular weights of parent pullulan. Drug showed slower release in vitro from the nanoparticles with higher molecular weights of parent pullulan, higher stability of the nanoparticles, and higher drug contents.

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