Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (38): 7082-7086.doi: 10.3969/j.issn.1673-8225.2010.38.013

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Influence of altered lipid metabolism in microencapsulated HepG2 cells on the mitochondrial function and protein synthesis

Xiao Jing 1,2, Zhang Ying1, Yu Wei-ting1, Guo Xin1, Wang Wei1, Ma Xiao-jun1   

  1. 1 Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian   116023, Liaoning Province, China; 2 Graduate School of the Chinese Academy of Sciences, Beijing   100039, China
  • Online:2010-09-17 Published:2010-09-17
  • Contact: Ma Xiao-jun, Investigator, Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning Province, China maxj@dicp.ac.cn
  • About author:Xiao Jing☆, Studying for doctorate, Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning Province, China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, China xiaoj@dicp.ac.cn
  • Supported by:
    the National Natural Science Foundation of China, No. 30970885*; the National Science and Technology Major Project of China, No. 2008ZX10002-019*

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Abstract:

BACKGROUND: The growth and production characteristics of animals, plants and microbial cells cultured in a special micro-environment, are different from those under normal physiological conditions or conventional culture environment, and this change achieves a relatively favorable micro-environment of the organisms under the premise of maintaining total macro-environment and conditions. Microencapsulated environment determines cell growth and metabolism behavior, and is closely related with function of microencapsulated cells.
OBJECTIVE: To study the influence of altered lipid metabolism occurred in microencapsulated hepatic cells on the mitochondrial function and protein synthesis capacity.
METHODS: Microencapsulated HepG2 cells were incubated in MEM with 1 μmol/L fluvastatin and cultured at 37 ℃ in an atmosphere with 5% volume fraction of CO2. The contents of total cholesterol, triglyceride, and albumin in the microencapsulated HepG2 cells were measured before and after fluvastatin intervention, as well as mitochondrial function. The growth of microencapsulated HepG2 cells was observed under phase-contrast microscopy.
RESULTS AND CONCLUSION: ①Total cholesterol and triglyceride were markedly increased in microencapsulated HepG2 cells with the increasing culture time (P < 0.05), and mitochondrial function declined (P < 0.05). ②Fluvastatin at 1 μmol/L reduced the total cholesterol levels in encapsulated HepG2 cells (P < 0.05), but there was no significant difference in the triglycerides level (P > 0.05). ③The inhibition of mitochondrial function and the reduction of albumin levels in microencapsulated cells can be relieved by fluvastatin (P < 0.05). Altered lipid metabolism in microencapsulated HepG2 cells, especially the increasing cholesterol synthesis, contributes to the mitochondrial function inhibition and protein synthesis decline.

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