Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (38): 7059-7064.doi: 10.3969/j.issn.1673-8225.2010.38.008

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Biocompatibility and bioactivity of an electrically conducting polymer, oxidized polypyrrole/polystyrene sulfate, for stimulation of neurite outgrowth in vitro

Zhou Yan1, Yu Xin-guang2, Yu Long1, Zhang Qing-wu3, Su Chong-de1, Xu Xue-jun1   

  1. 1 Department of Neurosurgery, Hospital Affiliated to Aeromedicine Institute of PLA, Beijing   100089, China; 2 Department of Neurosurgery, General Hospital of Chinese PLA, Beijing   100089, China; 3 China University of Mining and Technology, Xuzhou   221116, Jiangsu Province, China
  • Online:2010-09-17 Published:2010-09-17
  • Contact: Yu Xin-guang, Doctor, Chief physician, Professor, Department of Neurosurgery, General Hospital of Chinese PLA, Beijing 100089, China xinguang_yu@263.net
  • About author:Zhou Yan☆, Doctor, Attending physician, Department of Neurosurgery, Hospital Affiliated to Aeromedicine Institute of PLA, Beijing 100089, China zhouy1974@hotmail.com

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Abstract:

BACKGROUND: Synthesis of conductive polymers is flexible, the conductive polymer may show different and more suitable surface properties by changing the synthesis conditions.
OBJECTIVE: To investigate the biocompatibility of a conductive polymer-oxidized polypyrrole/polystyrene sulfate for stimulation of neurite outgrowth in vitro.
METHODS: The polypyrrole/polystyrene sulfate film was synthesized electrochemically and tested on electrochemical characters. The experiments were observed in four groups, including electrical stimulation, non-stimulation, blank control and solution control groups. The neurite outgrowth of pheochromocytoma cell line PC 12 was evaluated after in vitro electrical stimulation. The polypyrrole/polystyrene sulfate-polylactic acid-polyglycolic acid disks were fabricated and implanted in adult male Lewis rats, to evaluate both short-term and long-term inflammatory response, as well as the histocompatibility.
RESULTS AND CONCLUSION: Image analysis of in vitro culture demonstrated that PC-12 cells attached and differentiated equally well on both polypyrrole/polystyrene sulfate films and control groups. Qualitative observation of polypyrrole/polystyrene sulfate film matrix after electrical stimulation showed that, PC-12 cells cultured on polypyrrole/polystyrene sulfate films and subjected to an electrical stimulus showed a significant increase in neurite lengths compared with ones that were not subjected to electrical stimulation. In addition, the spreading morphous for PC-12 cells grown subjected to an electrical stimulus was greater than ones in control groups, but no cytotoxic reaction occurred. In vivo experiment proved no inflammatory response was induced by polypyrrole/polystyrene sulfate films. The polypyrrole/polystyrene sulfate film is a suitable material and shows good histocompatibility.

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