Abstract:

BACKGROUND: Preliminary study found that Dacron vascular prosthesis may inhibit anastomotic intimal and smooth muscle hyperplasia, reduce anastomotic stenosis and increase blood flow by grafting 2-methacryloyloxyethyl phosphorylcholine (MPC).
OBJECTIVE: To study the biocompatibility of Dacron vascular prosthesis coated with 2-MPC by ozonization in rabbit model.
METHODS: Dacron vascular prosthesis was grafted with MPC by ozonization and pruned into oval patches at 5 mm × 2 mm. Totally 24 New Zealand rabbits were processed into occluding the abdominal aorta and longitudinally incising the anterior wall of the abdominal aorta. Rabbits were divided into two groups randomly. The treated group was transplanted with Dacron prosthesis grafted MPC, while control group with Dacron prosthesis without MPC graft. At 1 day and 1, 2, 4 weeks postoperation, rabbits were killed for hematoxylin-eosin staining and Masson staining. Scanning electron microscopy was used to observe the intimal hyperplasia and blood cell adhesion on the surface of Dacron prosthesis.
RESULTS AND CONCLUSION: The surface of Dacron vascular prosthesis grafted MPC reduced neointimal proliferation compared with the control group. The thickness of intimal hyperplasia was not measured due to Dacron graft surface was not flat. Stratification of the neointima was found in the control groups at 2 weeks postoperation in Masson staining, and a great deal of cells infiltrated in the lower layer, not obvious in the superficial layer. No obvious stratification in neointima was found in the treated group until 4 weeks. Perhaps it had stratification. The lower lay was so thin than attachment to the Dacron graft surface was not obvious. It is believed that in the Dacron vascular prosthesis grafted MPC, effectively inhibit thrombosis, infiltration of inflammatory cells and deposition of fibrinogen, reduce hyperplasia of neointimal. Dacron vascular prosthesis coated with MPC significantly gets better biocompatibility.